Human BRAF Gene V600E Mutation
Product name
HWTS-TM007-Human BRAF Gene V600E Mutation Detection Kit(Fluorescence PCR)
Certificate
CE/TFDA
Epidemiology
More than 30 types of BRAF mutations have been found, of which about 90% are located in exon 15, where V600E mutation is considered to be the most common mutation, that is, thymine(T) at position 1799 in exon 15 is mutated to adenine (A), resulting in the replacement of valine (V) at position 600 by glutamic acid (E) in the protein product. BRAF mutations are commonly found in malignant tumors such as melanoma, colorectal cancer, thyroid cancer, and lung cancer. Understanding the mutation of BRAF gene has become the need to screen EGFR-TKIs and BRAF gene mutation-targeted drugs in clinical targeted drug therapy for the patients who can be benefited.
Channel
| FAM | V600E mutation, internal control |
Technical Parameters
|
Storage |
≤-18℃ |
|
Shelf-life |
9 months |
|
Specimen Type |
paraffin-embedded pathological tissue samples |
|
CV |
＜5.0% |
|
Ct |
≤38 |
|
LoD |
Use the kits to detect the corresponding LoD quality control. a) under 3ng/μL wild-type background, 1% mutation rate can be detected in the reaction buffer stably; b) under 1% mutation rate, the mutation of 1×103Copies/mL in the wild-type background of 1×105Copies/mL can be stably detected in the reaction buffer; c) the IC Reaction Buffer can detect the lowest detection limit quality control SW3 of the company internal control. |
|
Applicable Instruments: |
Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7300 Real-Time PCR
Systems, QuantStudio® 5 Real-Time PCR Systems LightCycler®480 Real-Time PCR system BioRad CFX96 Real-Time PCR System |
Work Flow
Recommended extraction reagents: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) manufactured by Tiangen Biotech(Beijing) Co., Ltd.
