11 Kinds of Respiratory Pathogen
Product name
HWTS-RT162A 11 Kinds of Respiratory Pathogen Nucleic Acid Detection Kit (Fluorescence PCR)
Channel
| 靶标 | Channel | |||
| 核酸反应液A | 核酸反应液B | 核酸反应液C | 核酸反应液D | |
| HI | Smet | MP | Leg | FAM |
| SP | PA | Bp | / | CY5 |
| KPN | ABA | Bpp | Cpn | ROX |
| 内参 | 内参 | 内参 | 内参 | VIC/HEX |
Epidemiology
Respiratory tract infections are an important class of diseases that pose a serious threat to human health, and studies have shown that most respiratory tract infections are caused by bacterial and or viral pathogens co-infecting the host, leading to increased severity of the disease and even death, so clarifying the pathogen allows for targeted treatment and improves the patient's survival rate [1,2].
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Sputum |
| Ct | HI、SP、KPN、PA、ABA、Smet：Ct≤33 BP、Bpp、MP、Cpn、Leg：Ct≤38 |
| CV | <5.0% |
| LoD | Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonas aeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia and Legionella pneumophila: 1000 CFU/mL；
Bordetella pertussis and Bacillus parapertussis: 500 CFU/mL; Mycoplasma pneumoniae and Chlamydia pneumoniae: 200 copies/mL. |
| Specificity | The cross-reactivity test results showed that there was no cross reaction between this kit and Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii and human genomic nucleic acids. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Add 200µL of physiological saline to the processed precipitate, and the subsequent steps should be carried out in accordance with the instructions. The recommended elution volume is 100 µL.
