14 Kinds of Respiratory Pathogens Combined
Product name
HWTS-RT159B 14 Kinds of Respiratory Pathogens Combined Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Respiratory tract infection is the most common disease in humans, which can occur in any gender, age and region. It is one of the leading causes of morbidity and mortality in the population worldwide[1]. Common respiratory pathogens include novel coronavirus, Influenza A virus, Influenza B virus, Respiratory syncytial virus, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Streptococcus pneumoniae, etc[2,3].
Channel
| Well Position | Reaction Solution Name | Pathogens to be Detected |
| 1 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 2 | Master Mix B | Adv, hMPV, MP, Cpn |
| 3 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Mix D | CoV, EV, SP, internal control |
| 5 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 6 | Master Mix B | Adv, hMPV, MP, Cpn |
| 7 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Mix D | CoV, EV, SP, internal control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 9 months |
| Specimen Type | Oropharyngeal swab、Nasopharyngeal swab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Copies/mL |
| Specificity | The cross-reactivity test results showed that there was no cross reaction between this kit and Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii and human genomic nucleic acids. |
| Applicable Instruments | SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.)Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The extracted sample volume is 200µL. Subsequent steps should be performed according to the instructions for use of this extraction reagent. The recommended elution volume is 80µL.
