17 Types of HPV (16/18/6/11/44 Typing)
Product name
HWTS-CC015 17 Types of Human Papillomavirus (16/18/6/11/44 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Cervical cancer is one of the most common malignant tumors in the female reproductive tract. It has been shown that persistent HPV infection and multiple infections are one of major causes of cervical cancer. Currently there is still a lack of generally accepted effective treatments for cervical cancer caused by HPV. Therefore, early detection and prevention of cervical infection caused by HPV are the keys to prevention of cervical cancerization. Establishment of simple, specific and rapid diagnostic tests for pathogens is of great significance for clinical diagnosis of cervical cancer.
Channel
| PCR-Mix1 | FAM | 18 |
| VIC/HEX |
16 |
|
| ROX |
31，33，35，39，45，51，52，56，58，59，66，68 |
|
| CY5 | Internal control |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX |
11 |
|
| ROX |
44 |
|
| CY5 | Internal control |
Technical Parameters
| Storage |
-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Urine sample, female cervical swab sample, female vaginal swab sample |
| Ct | ≤28 |
| LoD | 300Copies/mL |
| Specificity | There is no cross-reactivity with Ureaplasma urealyticum, Chlamydia trachomatis of reproductive tract, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold , Gardnerella and other HPV types not covered by the kit. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Add 200μL of normal saline to resuspend the pellet in step 2.1, and then the extraction should be conducted according to the instructions for use of this extraction reagent. The recommended elution volume is 80μL.
Recommended extraction reagent: QIAamp DNA Mini Kit (51304) or Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Add 200μL of normal saline to resuspend the pellet in step 2.1, and then the extraction should be conducted according to the instructions for use of this extraction reagent. The extracted sample volume of samples are all 200μL, and the recommended elution volume is 100μL.
Recommended extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Add 200μL of sample release reagent to resuspend the pellet in step 2.1, and then the extraction should be conducted according to the instructions for use of this extraction reagent.
