ALDH Genetic Polymorphism
Product name
HWTS-GE015 ALDH Genetic Polymorphism Detection Kit (ARMS -PCR)
Epidemiology
ALDH2 gene (acetaldehyde dehydrogenase 2), locates on human chromosome 12. ALDH2 has esterase, dehydrogenase and reductase activity at the same time. Studies have shown that ALDH2 is a metabolic enzyme of nitroglycerin, which converts nitroglycerin into nitric oxide, thereby relaxing blood vessels and improving blood flow disorders. However, there are polymorphisms in the ALDH2 gene, which are mainly concentrated in East Asia. The wild-type ALDH2*1/*1 GG has strong metabolic ability, while the heterozygous type has only 6% of the wild-type enzyme activity, and the homozygous mutant type has almost zero enzyme activity, with metabolism extremely weak and cannot achieve the desired effect, thus causing harm to the human body.
Channel
| FAM | ALDH2 |
| ROX |
Internal Control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | EDTA anticoagulated blood |
| CV | <5.0％ |
| LoD | 103Copies/mL |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Recommended extraction reagents: use Blood Genome DNA Extraction Kit (DP318) by Tiangen Biotech (Beijing) Co.,Ltd. or Blood Genome Extraction Kit (A1120) by Promega to extract EDTA anticoagulated blood Genomic DNA.
Recommended extraction reagents: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The extraction should be conducted according to the instructions strictly. The recommended elution volume is 100μL.
