Best Price on Rapid Combo Test Dengue - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test

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Our team through qualified training. Skilled professional knowledge, powerful sense of support, to satisfy the support desires of consumers for Human Egfr Gene 29 Mutations Detection Kit (Fluorescence Pcr), Rotavirus Antigen, Hepatitis C, We take quality as the foundation of our success. Thus, we focus on the manufacture of the best quality products. A strict quality management system has been created to ensure the quality of the products.
Best Price on Rapid Combo Test Dengue - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)
Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

Certificate

CE

Epidemiology

Dengue fever (DF), which is induced by denguevirus (DENV) infection, is one of the most epidemic arbovirus infectious diseases. DENV belongs to flavivirus under flaviviridae, and can be classified into 4 serotypes according to surface antigen. Its transmission medium includes Aedes aegypti and Aedes albopictus, prevalent mainly in tropical and subtropical areas.

Clinical manifestations of DENV infection mainly include headache, fever, weakness, enlargement of lymph node, leukopenia and etc., and bleeding, shock, hepatic injury or even death in severe cases. In recent years, climate change, urbanization, quick development of tourism and other factors have provided more rapid and convenient conditions for transmitting and spreading of DF, leading to constant expansion of epidemic area of DF.

Features

● Multiplex PCR Amplification Technology.
● Sub-Package: Safer and easier.
● High sensitivity: 500 Copies/mL.
● High specificity: No cross reactivity with common pathogens causing febrile and hemorrhagic diseases.

Channel

FAM: Dengue Virus I
VIC(HEX): Dengue Virus II
ROX: Dengue Virus III
CY5: Dengue Virus IV

PCR Amplification Conditions Setting

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 30mins No
2 1 cycle 95℃ 15mins No
3 40 cycles 95℃ 15s No
60℃ 45s Yes

Technical Parameters

Storage
Liquid ≤-18℃ in dark; lyophilization:≤30℃ in dark
Shelf-life 12 months
Specimen Type Fresh Serum
Ct ≤38
CV ≤5.0%
LoD 500 Copies/mL

Specificity: Perform cross reaction tests of Japanese encephalitis virus, Forest encephalitis virus, severe fever with thrombocytopenia syndrome, Xinjiang hemorrhagic fever, hantaan virus, hepatitis C virus, influenza A virus, influenza B virus and etc. No cross reaction is detected.

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
ABI 7500 Fast Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.
BioRad CFX96 Real-Time PCR System.
BioRad CFX Opus 96 Real-Time PCR System.

Main Components

Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

Component Catalogue Number Quantity Component Description
HWTS-FE034A HWTS-FE034B
Specification(20 tests/kit) Specification(50 tests/kit)
DENV Reaction Buffer 394μL/vial 985μL/vial 1 vial Dengue virus I/II/III/IV primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc.
DENV Enzyme Mix 6μL/vial 15μL/vial 1 vial Reverse transcriptase, etc.
DENV Positive Control 400μL/vial 1 mL/vial 1 vial Mixture of DENV I/II/III/IV templates
DENV Blank Control 400μL/vial 1 mL/vial 1 vial DNase/RNase free H2O

Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

Component Character Catalogue Number Component Description
HWTS-FE004B HWTS-FE004C
Specification/Quantity(20 tests/kit) Specification/Quantity (50 tests/kit)
DENV Reaction Buffer lyophilized 1 bottle 1 bottle dengue virus I/II/III/IV primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc.
DENV Positive Control lyophilized 1 vial 1 vial Mixture of DENV I/II/III/IV templates
DENV Blank Control Liquid 1 vial, 600μL/vial 1 vial, 600μL/vial DNase/RNase free H2O
Reconstituted Solution Liquid 1 vial, 1mL/vial 1 vial, 1.45mL/vial DNase/RNase free H2O, etc.

Total PCR Solution

Dengue Virus I II III IV Nucleic Acid Detection Kit6

Reference

[1] Shu P Y, Chang S F, Kuo Y C, et al. Development of Group- and Serotype-Specific One-Step Sybr Green I-Based Real-Time Reverse Transcription-PCR Assay for Dengue Virus[J]. Journal of Clinical Microbiology, 2003, 41(6):2408-2416.
[2] Paudel D, Jarman R , Limkittikul K, et al. Comparison of real-time SYBR green dengue assay with real-time taqman RT-PCR dengue assay and the conventional nested PCR for diagnosis of primary and secondary dengue infection[J]. North American Journal of Medical Sciences, 2011, 3(10):478-485.
[3] Waggoner J J, Ballesteros G, Gresh L, et al. Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection[J]. Journal of Clinical Virology the Official Publication of the Pan American Society for Clinical Virology, 2016:57-61.
[4] Yao J X, Zhao W, Zhang L, et al. Research of Real-Time Fluorescence PCR Detection on Dengue Virus[J]. West China Medical Journal, 2009.


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Best Price on Rapid Combo Test Dengue - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Best Price on Rapid Combo Test Dengue - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Best Price on Rapid Combo Test Dengue - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Best Price on Rapid Combo Test Dengue - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Best Price on Rapid Combo Test Dengue - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures


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  • Products and services are very good, our leader is very satisfied with this procurement, it is better than we expected,
    5 Stars By Roberta from Slovakia - 2018.11.28 16:25
    Managers are visionary, they have the idea of mutual benefits, continuous improvement and innovation, we have a pleasant conversation and Cooperation.
    5 Stars By Ada from Swedish - 2017.10.13 10:47
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