Bottom price Hpv - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test

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Bottom price Hpv - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test Detail:

Product name

28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR )

Certificate

CE

Epidemiology

Cervical cancer is one of the most common malignant tumors in the female reproductive tract. The studies have shown that persistent infection and multiple infections of human papillomavirus is one of the important cause of cervical cancer. At present, there is still a lack of recognized effective treatment methods for HPV. Therefore, early detection and early prevention of cervical HPV is the key to blocking canceration. The establishment of a simple, specific and rapid pathogenic diagnostic method is of great significance in the clinical diagnosis of cervical cancer.

Features

● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 25 Copies/reaction
● High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.
● Convenient: One test for multiple results.

Channel

S/N Channel Type
PCR-Mix1 FAM 16, 18, 31, 56
VIC(HEX) Internal Control
CY5 45, 51, 52, 53
ROX 33, 35, 58, 66
PCR-Mix2 FAM 6, 11, 54, 83
VIC(HEX) 26, 44, 61, 81
CY5 40, 42, 43, 82
ROX 39, 59, 68, 73

PCR Amplification Conditions Setting

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 2 mins No
2 1 cycle 95℃ 10 mins No
3 10 cycle 95℃ 15 s No
62℃ 20 s No
4 30 cycle 95℃ 15 s No
58℃ 30 s Yes

Technical Parameters

Storage ≤-18℃ in dark  
Shelf-life 12 months
Specimen Type Cervical exfoliated cells 
Ct ≤28
CV ≤5.0%
LoD 25 Copies/reaction
Specificity No cross-reactivity with common reproductive tract pathogens (such as ureaplasma urealyticum, genital tract chlamydia trachomatis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella and other HPV types not covered in the kit, etc).
Applicable Instruments It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems
ABI 7500 Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems
LightCycler®480 Real-Time PCR Systems
LineGene 9600 Plus Real-Time PCR Detection Systems  
MA-6000 Real-Time Quantitative Thermal Cycler

Main Components

Catalogue Number Component (50 tests/kit) Specification Quantity Component Description
HWTS-CC003A HPV WELL1 1.3mL/vial 1 vial Mixture of specific primers and probes of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66
HPV WELL2 1.3mL/vial 1 vial Mixture of specific primers and probes of HPV6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83
HPV Enzyme Mix 55uL/vial 1 vial Mixture of Taq DNA polymerase, Uracil-DNA Glycosylase and glycerin
HPV Positive Control WELL1 150μL/vial 1 vial Mixture of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66 templates and Internal Control template
HPV Positive Control WELL2 150μL/vial 1 vial Mixture of 6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83 templates
HPV Blank Control 300μL/vial 1 vial RNase/DNase free H2O

Total PCR Solution

Option 1.

Fluorescence PCR3

Option 2.

Fluorescence PCR4


Product detail pictures:

Bottom price Hpv - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test detail pictures

Bottom price Hpv - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test detail pictures

Bottom price Hpv - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test detail pictures


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    5 Stars By Lorraine from Tunisia - 2018.09.29 13:24
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