China Manufacturer for Respiratory Viral Panel - AdV Universal and Type 41 Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test

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China Manufacturer for Respiratory Viral Panel - AdV Universal and Type 41 Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

Adenovirus Universal and Type 41 Nucleic Acid Detection Kit (Fluorescence PCR) 

Certificate

CE

Packaging Size

1. Liquid product: 50 tests/kit

2. Freeze-dried product (penicillin vial): 20 tests/kit, 50 tests/kit

3. Freeze-dried product (8-tube strips): 48 tests/kit

Intended Use

This kit is used for in vitro qualitative detection of adenovirus nucleic acid in nasopharyngeal swabs, throat swabs and stool samples.

Epidemiology

Human adenovirus (HAdV) belongs to the genus Mammalian adenovirus, which is a double-stranded DNA virus without envelope. Adenoviruses that have been found so far include 7 subgroups (A-G) and 67 types, of which 55 serotypes are pathogenic to humans. Among them, could leading to the respiratory tract infections are mainly group B (Types 3, 7, 11, 14, 16, 21, 50, 55), Group C (Types 1, 2, 5, 6, 57) and Group E (Type 4), and could leading to intestinal diarrhea infection is Group F (Types 40 and 41)[1-8].

Respiratory diseases caused by respiratory tract infections of human body account for 5%~15% of global respiratory diseases, and 5%~7% of global childhood respiratory diseases[9], which could also infect the gastrointestinal tract, urethra, bladder, eyes, and liver, etc. Adenovirus is endemic in a wide range of areas and can be infected all year round, especially in crowded areas, which are prone to local outbreaks, mainly in schools and military camps.

Features

Multiplex PCR Amplification Technology

Internal control: Fully monitor the experimental process to ensure the quality of Experiments.

High sensitivity: 300Copies/mL

High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.

Rapid: Rapid PCR amplification, test results can be obtained in 40 minutes.

Channel

FAM: Adenovirus universal nucleic acid

ROX: Adenovirus type 41 nucleic acid

VIC (HEX): Internal control

PCR Amplification Conditions Setting

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 2mins No
2 1 cycle 95℃ 1min No
3 40 cycles 95℃ 5s No
55℃ 15s Yes

Technical Parameters

Storage:

Liquid: ≤-18℃ In dark  

Lyophilization: ≤30℃ In dark

Shelf-life: 12 months

Specimen Type: Nasopharyngeal swab, Throat swab, Stool samples

Ct: ≤38

CV: ≤5.0%

LoD: 300Copies/mL

Specificity: Use this kit to detect and there is no cross-reactivity with other respiratory pathogens (such as Influenza A virus, Influenza B virus, Respiratory syncytial virus, Parainfluenza virus, Rhinovirus, Human metapneumovirus, etc.) or bacteria (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, etc.) and common gastrointestinal pathogens Group A rotavirus, Escherichia coli, etc.

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.

ABI 7500 Real-Time PCR Systems

ABI 7500 Fast Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

LightCycler®480 Real-Time PCR Systems

LineGene 9600 Plus Real-Time PCR Detection Systems

MA-6000 Real-Time Quantitative Thermal Cycler 

Main Components

Liquid product

Catalogue Number Component (50 tests/kit) Specification Quantity Component Description
HWTS-RT112A ADV Reaction Buffer  1.25mL /vial 1 vial Amplification reaction reagent, adenovirus universal primer probe, adenovirus type 41 primer probe, internal control primer probe, etc.
ADV Positive Control 250μL/vial  1 vial Adenovirus and internal control plasmids mixture.
ADV Blank Control 250μL/vial 1 vial DNase/RNase free H2O

Lyophilized product

Component Character Catalogue Number Component Description
    HWTS-RT112B HWTS-RT112C HWTS-RT112Z  
    Specification/Quantity

(20 tests/kit)

Specification/Quantity

(50 tests/kit)

Specification/Quantity

(48 tests/kit)

 
ADV Reaction Buffer lyophilized 1 bottle 1 bottle 8-tube strips, 6 strips Amplification reaction reagent, adenovirus universal primer probe, adenovirus type 41 primer probe, internal control primer probe, etc.
ADV Positive Control lyophilized 1 vial 1 vial 1 vial Adenovirus and internal control plasmids mixture.
ADV Blank Control Liquid 1 vial, 250μL/vial 1 vial, 250μL/vial 1 vial, 250μL/vial DNase/RNase free H2O
ADV Reconstituted Solution Liquid 1 vial, 650μL/vial 1 vial, 1.25mL/vial 1 vial, 1.21mL/vial DNase/RNase free H2O, etc.

 

Total PCR Solution

Option 1.

高清图1

Option 2. 

高清图2

Reference

[1] SunB, HeH, WangZ, et al. Emergent severe acute respiratory distress syndrome caused by adenovirus type 55 in immunocompetent adults in 2013: a prospective observational study[J]. Crit Care, 2014, 18(4):456.

[2] CaoB, HuangGH, PuZH, et al. Emergence of community-acquired adenovirus type 55 as a cause of community-onset pneumonia[J]. Chest, 2014, 145(1):79-86.

[3] ABBAS K Z, LOMBOS E, DUVVURI V R, et al. Temporal changes in respiratory adenovirus serotypes circulating in the greater Toronto area, Ontario, during December 2008 to April 2010[J]. Virology Journal, 2013, 10(1): 15. DOI: 10.1186/1743-422X-10-15.

[4] YEUNG R, ESHAGHI A, LOMBOS E, et al. Characterization of culture-positive adenovirus serotypes from respiratory specimens in Toronto, Ontario, Canada: September 2007- June 2008[J]. Virology Journal, 2009,6(1): 11. DOI: 10.1186/1743-422x-6-11.

[5] NODA M, YOSHIDA T, SAKAGUCHI T, et al. Molecular and epidemiological analyses of human adenovirus type 7 strains isolated from the 1995 nation wide outbreak in Japan [J]. Journal of Clinical Microbiology, 2002, 40(1): 140-145.

[6] BARRAZA E M, LUDWIG S L, GAYDOS J C, et al. Reemergence of Adenovirus Type 4 Acute Respiratory Disease in Military Trainees: Report of an Outbreak during a Lapse in Vaccination[J]. Journal of Infectious Diseases, 1999, 179(6): 1531-1533.

[7] MEI Y F, SKOG J, LINDMAN K, et al. Comparative analysis of the genome organization of human adenovirus 11, a member of the human adenovirus species B, and the commonly used human adenovirus 5 vector, a member of species C[J]. Journal of General Virology, 2003, 84(8): 2061-2071.

[8] PRIMO D, PACHECO G T, TIMENETSKY M. Surveillance and molecular characterization of human adenovirus in patients with acute gastroenteritis in the era of rotavirus vaccine, Brazil, 2012-2017[J]. Journal of Clinical Virology, 2018, 109: 35-40.

[9] Ghebremedhin B. Human adenovirus: Viral pathogen with increasing importance[J]. European Journal of Microbiology and Immunology, 2014, 4(1): 26-33.

实物图1
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Product detail pictures:

China Manufacturer for Respiratory Viral Panel - AdV Universal and Type 41 Nucleic Acid Detection Kit (Fluorescence PCR)  – Macro & Micro-Test detail pictures

China Manufacturer for Respiratory Viral Panel - AdV Universal and Type 41 Nucleic Acid Detection Kit (Fluorescence PCR)  – Macro & Micro-Test detail pictures

China Manufacturer for Respiratory Viral Panel - AdV Universal and Type 41 Nucleic Acid Detection Kit (Fluorescence PCR)  – Macro & Micro-Test detail pictures

China Manufacturer for Respiratory Viral Panel - AdV Universal and Type 41 Nucleic Acid Detection Kit (Fluorescence PCR)  – Macro & Micro-Test detail pictures

China Manufacturer for Respiratory Viral Panel - AdV Universal and Type 41 Nucleic Acid Detection Kit (Fluorescence PCR)  – Macro & Micro-Test detail pictures

China Manufacturer for Respiratory Viral Panel - AdV Universal and Type 41 Nucleic Acid Detection Kit (Fluorescence PCR)  – Macro & Micro-Test detail pictures


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  • The product manager is a very hot and professional person, we have a pleasant conversation, and finally we reached a consensus agreement.
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