China OEM Eb Virus Nucleic Acid Detection Kit(Fluorescence Pcr) - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test
China OEM Eb Virus Nucleic Acid Detection Kit(Fluorescence Pcr) - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test Detail:
Product name
EB Virus Nucleic Acid Detection Kit(Fluorescence PCR)
Certificate
CE
Epidemiology
EBV (Epstein-barr virus), or human herpesvirus type 4, is a common human herpesvirus[1]. In recent years, a large number of studies have proved that EBV is associated with occurrence and development of nasopharyngeal cancer, Hodgkin’s disease, T/Natural killer celllymphoma, Burkitt’s lymphoma, breast cancer, gastric cancer and other malignant tumors. And it is also closely associated with post-transplantlymphoproliferative disorders, post-transplant smooth muscle tumor and acquired immunedeficiency syndrome(AIDS) related lymphoma, multiple sclerosis, primary central nervous system lymphoma or leiomyosarcoma[2-4].
Features
● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 500Copies/mL.
● Convenient: One test for multiple results.
Channel
FAM: EBV
VIC (HEX): Internal control
PCR Amplification Conditions Setting
Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
1 | 1 cycle | 50℃ | 2mins | No |
2 | 1 cycle | 95℃ | 5mins | No |
3 | 40 cycles | 95℃ | 15s | No |
58℃ | 30s | Yes |
Technical Parameters
Storage | ≤-18℃ In dark |
Shelf-life | 12 months |
Specimen Type | Whole blood, Plasma, Serum |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 500Copies/mL |
Specificity: It has no cross-reactivity with other pathogens (such as human herpesvirus 1, 2, 3, 6, 7, 8, hepatitis B virus, cytomegalovirus, influenza A, etc.) or bacteria (Staphylococcus aureus, Candida albicans, etc.)
Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.
Main Components
Catalogue Number | Component (50 tests/kit) | Specification | Quantity | Component Description |
HWTS-OT061A | EB Reaction Buffer | 1.175mL/vial | 1 vial | Amplification reaction reagents, EB virus primer probes, internal reference primer probes, etc. |
EB Enzyme Mix | 75μL/vial | 1 vial | Taq enzyme, UDG enzyme, etc. | |
EB Positive Control | 600μL/vial | 1 vial | Mixed solution of viral target gene and internal reference | |
EB Blank Control | 600μL/vial | 1 vial | DNase/RNase free H2O |
Total PCR Solution
Reference
[1] ohen JI, Jaffe ES, Dale JK, et al. Characterization and treatment of chronic active Epstein-Barr virus disease: a 28-year experience in the United States[J]. Blood, 2011, 117(22): 5835-5849.
[2] Jahangiryan A, Kheirandish M, Samiee S, et al. Determination of Epstein-Barr virus (EBV) incidence in umbilical cord blood (UCB) and assessment of virus DNA via Real-time PCR[J]. Annals of Cancer Research and Therapy, 2021, 29(1):114-120.
[3] Petersson F. Nasopharyngeal carcinoma:a review[J].Semin Diagn Pathol, 2015, 32(1):54-73. [4]Chen J N, He D, Tang F, et al. Epstein-Barr virus-associated gastric carcinoma:a newly defined entity[J]. J Clin Gastroenterol, 2012, 46(4):262-271.
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With our abundant experience and considerate products and services, we have been recognized to be a reputable supplier for a lot of global consumers for China OEM Eb Virus Nucleic Acid Detection Kit(Fluorescence Pcr) - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test , The product will supply to all over the world, such as: Hungary, Russia, South Africa, As an experienced factory we also accept customized order and make it same as your picture or sample specifying specification and customer design packing. The main goal of the company is to live a satisfactory memory to all the customers, and establish a long term win-win business relationship. For more information, please contact us. And It is our great pleasure if you like to have a personally meeting in our office.
By Muriel from Miami - 2018.05.13 17:00
The accounts manager made a detailed introduction about the product, so that we have a comprehensive understanding of the product, and ultimately we decided to cooperate.
By Carlos from Philippines - 2017.09.28 18:29