China OEM Hpv Types - 18 Types of High-risk Human Papilloma Virus Nucleic Acid Detection Kit – Macro & Micro-Test

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China OEM Hpv Types - 18 Types of High-risk Human Papilloma Virus Nucleic Acid Detection Kit – Macro & Micro-Test Detail:

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18 Types of High-risk Human Papilloma Virus Nucleic Acid Detection Kit (Fluorescence PCR)

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Epidemiology

Cervical cancer is one of the most common malignant tumors in the female reproductive tract. Studies have shown that persistent infection and multiple infections of human papillomavirus are one of the important causes of cervical cancer.

Reproductive tract HPV infection is common among women with sexual life. According to statistics, 70% to 80% of women may have HPV infection for once at least in their lifetime, but most infections are self-limiting, and more than 90% of infected women will develop an effective immune response that could clear the infection between 6 and 24 months without any long-term health intervention. Persistent high-risk HPV infection is the main cause of cervical intraepithelial neoplasia and cervical cancer.

The worldwide study results showed that the presences of high-risk HPV DNA were detected in 99.7% of cervical cancer patients. Therefore, the early detection and prevention of cervical HPV are the key to blocking canceration. The establishment of a simple, specific and rapid pathogenic diagnostic method is of great significance in the clinical diagnosis of cervical cancer.

Features

● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.
● High sensitivity: 25 copies/reaction.
● Convenient: One test for multiple results.

Channel

FAM	HPV 18
VIC (HEX)	HPV 16
ROX	HPV 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82
CY5	Internal control

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	2 mins	No
2	1 cycle	95℃	10 mins	No
3	10 cycle	95℃	15 s	No
3	10 cycle	62℃	20 s	No
4	30 cycle	95℃	15 s	No
4	30 cycle	58℃	30 s	Yes

Technical Parameters

Storage	≤-18℃ in dark
Shelf-life	24 months
Specimen Type	Cervical exfoliated cells
Ct	≤28
CV	≤5.0％
LoD	25 Copies/reaction
Specificity	No cross-reactivity with common reproductive tract pathogens (such as ureaplasma urealyticum, genital tract chlamydia trachomatis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella and other HPV types not covered in the kit, etc).
Applicable Instruments	It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems
ABI 7500 Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems
LightCycler®480 Real-Time PCR Systems
LineGene 9600 Plus Real-Time PCR Detection Systems
MA-6000 Real-Time Quantitative Thermal Cycler

Main Components

Catalogue Number	Component (50 tests/kit)	Specification	Quantity	Component Description
HWTS-CC011A	18 HPV Reaction Buffer	1.25mL/vial	1 vial	Amplification reaction reagents and kinds of primers, probes
	18 HPV Positive Control	600μL/vial	1 vial	Mixture of HPV 16, 18, 33 and their internal control plasmids
	18 HPV Blank Control	600μL/vial	1 vial	DNase/RNase free H2O

Total PCR Solution

Option 1.
1. Sampling

Option

2. Nucleic acid extraction

2.Nucleic acid extraction

3. Add samples to the machine

3.Add samples to the machine

Option 2.
1. Sampling

Option

2. Extraction-free

2.Extraction-free

3. Add samples to the machine

3.Add samples to the machine`

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18 Types Of High-Risk Hpv Nucleic Acid Detection Kit