China wholesale Vibrio Cholerae O1 And Enterotoxin Gene Nucleic Acid Detection Kit (Fluorescence Pcr) - Macro & Micro-Test Sample Release Reagent – Macro & Micro-Test
China wholesale Vibrio Cholerae O1 And Enterotoxin Gene Nucleic Acid Detection Kit (Fluorescence Pcr) - Macro & Micro-Test Sample Release Reagent – Macro & Micro-Test Detail:
Product name
Macro & Micro-Test Sample Release Reagent
Certificate
CE, FDA, NMPA
Features
Rapid: only 5 mins.
Easy: only 3 steps (1.add the sample 2.Mix upside and down 10 times 3. Leave it for 5 mins).
Main components
Name | Main components | Component specifications | Quantity |
Sample Release reagent | Dithiothreitol, sodium dodecyl sulfate (SDS), RNase inhibitor, surfactant, purified water | 0.5mL/Vial | 50 Vial |
Note: Components in different batches of kits are not interchangeable.
Storage conditions and shelf life
Store and transport at room temperature. The shelf life is 24 months.
Applicable instruments
Instruments and equipment during sample processing, such as pipettes, vortex mixers, water baths, etc.
Sample requirements
Freshly collected oropharyngeal swabs, nasopharyngeal swabs.
Precision
When this kit is used for extraction from the in-house precision reference CV for 10 replicates, the coefficient of variation (CV, %) of Ct value is not more than 10%.
Inter-batch difference
When the in-house precision reference is tested on three batches of kits under trial production upon repeated extraction and, the coefficient of variation (CV, %) of Ct value is not more than 10%.
Performance comparison
● Extraction efficiency comparsion
Efficiency comparsion of magnetic beads method and sample releaser |
||||
concentration |
magnetic beads method |
sample releaser |
||
orfab |
N |
orfab |
N |
|
20000 |
28.01 |
28.76 |
28.6 |
29.15 |
2000 |
31.53 |
31.9 |
32.35 |
32.37 |
500 |
33.8 |
34 |
35.25 |
35.9 |
200 |
35.25 |
35.9 |
35.83 |
35.96 |
100 |
36.99 |
37.7 |
38.13 |
undet |
The extraction efficiency of sample releaser was similar to that of magnetic beads method, and the concentration of pathogen could be 200Copies/mL.
● CV value comparison
Repeatability of sample releaser extraction |
||
concentration:5000Copies/mL |
ORF1ab |
N |
30.17 |
30.38 |
|
30.09 |
30.36 |
|
30.36 |
30.26 |
|
30.03 |
30.48 |
|
30.14 |
30.45 |
|
30.31 |
30.16 |
|
30.38 |
30.7 |
|
30.72 |
30.79 |
|
CV |
0.73% |
0.69% |
When tested at 5,000 copies /mL, the CV of orFab and N was 0.73% and 0.69%, respectively.
Product detail pictures:
Related Product Guide:
We insist on the principle of development of 'High quality, Efficiency, Sincerity and Down-to-earth working approach' to provide you with excellent service of processing for China wholesale Vibrio Cholerae O1 And Enterotoxin Gene Nucleic Acid Detection Kit (Fluorescence Pcr) - Macro & Micro-Test Sample Release Reagent – Macro & Micro-Test , The product will supply to all over the world, such as: Kuwait, French, Leicester, Our company regards reasonable prices, high quality,efficient production time and good after-sales service as our tenet. We hope to cooperate with more customers for mutual development and benefits in future. Welcome to contact us.
By Astrid from Sao Paulo - 2017.10.27 12:12
This is the first business after our company establish, products and services are very satisfying, we have a good start, we hope to cooperate continuous in the future!
By Diego from Victoria - 2018.06.12 16:22