Chlamydia Trachomatis, Neisseria Gonorrhoeae and Trichomonas vaginalis
Product name
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae and Trichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Chlamydia trachomatis (CT) is a kind of prokaryotic microorganism that is strictly parasitic in eukaryotic cells. Chlamydia trachomatis is divided into A-K serotypes according to the serotype method. Urogenital tract infections are mostly caused by trachoma biological variant D-K serotypes, and males are mostly manifested as urethritis, which can be relieved without treatment, but most of them become chronic, periodically aggravated, and can be combined with epididymitis, proctitis, etc.
Channel
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Trichomonal vaginitis |
| VIC/HEX | Internal Control |
Technical Parameters
| Storage |
-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Female cervical swab，Female vaginal swab，Male urethral swab |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400 Copies/mL |
| Specificity | There is no cross-reactivity with other STD infection pathogens outside the detection range of the test kit, such as Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus type 1, Herpes simplex virus type 2, Candida albicans, etc. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
Recommended extraction reagent: Pipette 1mL of the sample to be tested to a 1.5mL of DNase/RNase-free centrifuge tube, centrifuge at 12000rpm for 3 minutes, discard the supernatant and keep the precipitate. Add 200µL of normal saline to the precipitate to resuspend. Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The extraction should be performed according to the instruction for use. The extracted sample volume is 200µL, and the recommended elution volume is 80µL.
Option 2.
Recommended extraction reagent: Nucleic Acid Extraction or Purification Kit (YDP302). The extraction should be performed strictly according to the instruction for use. The recommended elution volume is 80µL.
