Chlamydia Trachomatis Nucleic Acid
Product Name
HWTS-UR001A-Chlamydia Trachomatis Nucleic Acid Detection Kit (Fluorescence PCR)
Intended Use
This kit is used for qualitative detection of Chlamydia trachomatis nucleic acid in male urine, male urethral swab, and female cervical swab samples.
Epidemiology
Chlamydia trachomatis (CT) is a kind of prokaryotic microorganism that is strictly parasitic in eukaryotic cells. Chlamydia trachomatis is divided into A-K serotypes according to the serotype method. Urogenital tract infections are mostly caused by trachoma biological variant D-K serotypes, and males are mostly manifested as urethritis, which can be relieved without treatment, but most of them become chronic, periodically aggravated, and can be combined with epididymitis, proctitis, etc. Females can be caused with urethritis, cervicitis, etc., and more serious complications of salpingitis.
Epidemiology
FAM: Chlamydia trachomatis (CT)·
VIC(HEX): Internal Control
PCR Amplification Conditions Setting
Step |
Cycles |
Temperature |
Time |
Collect Fluorescent Signals or Not |
1 |
1 cycle |
50℃ |
5mins |
No |
2 |
1 cycle |
95℃ |
10mins |
No |
3 |
40 cycles |
95℃ |
15 secs |
No |
4 |
58℃ |
31 secs |
Yes |
Technical Parameters
Storage |
≤-18℃ In dark |
Shelf-life |
12 months |
Specimen Type | Male urethral secretions, Female cervical secretions, Male urine |
Ct |
≤38 |
CV | <5.0% |
LoD | 400Copies/mL |
Specificity |
There is no cross-reactivity for detecting other STD-infected pathogens by this kit, such as Treponema pallidum, Neisseria gonorrhoeae, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, etc., which are outside the detection range of kit. |
Applicable Instruments |
It can match the mainstream fluorescent PCR instruments on the market. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |