Dengue Virus I/II/III/IV Nucleic Acid
Product name
HWTS-FE034-Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)
HWTS-FE004-Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)
Certificate
CE
Epidemiology
Dengue fever (DF), which is induced by denguevirus (DENV) infection, is one of the most epidemic arbovirus infectious diseases. DENV belongs to flavivirus under flaviviridae, and can be classified into 4 serotypes according to surface antigen. Its transmission medium includes Aedes aegypti and Aedes albopictus, prevalent mainly in tropical and subtropical areas.
Clinical manifestations of DENV infection mainly include headache, fever, weakness, enlargement of lymph node, leukopenia and etc., and bleeding, shock, hepatic injury or even death in severe cases. In recent years, climate change, urbanization, quick development of tourism and other factors have provided more rapid and convenient conditions for transmitting and spreading of DF, leading to constant expansion of epidemic area of DF.
Channel
FAM | Dengue Virus I |
VIC(HEX) | Dengue Virus II |
ROX | Dengue Virus III |
CY5 | Dengue Virus IV |
Technical Parameters
Storage | Liquid: ≤-18℃ in dark; lyophilization:≤30℃ in dark |
Shelf-life | Liquid: 9 months; lyophilization:12 months |
Specimen Type | Fresh Serum |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 500 Copies/mL |
Specificity | Perform cross reaction tests of Japanese encephalitis virus, Forest encephalitis virus, severe fever with thrombocytopenia syndrome, Xinjiang hemorrhagic fever, hantaan virus, hepatitis C virus, influenza A virus, influenza B virus and etc. No cross reaction is detected |
Applicable Instruments | It can match the mainstream fluorescent PCR instruments on the market. SLAN-96P Real-Time PCR Systems ABI 7500 Real-Time PCR Systems ABI 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |