Discount Price Urinary Reproduction - Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test

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Discount Price Urinary Reproduction - Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR)
Freeze-dried Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR)

Certificate

CE, FDA

Epidemiology

Group B Streptococcus (GBS), also known as streptococcus agalactiae, is a gram-positive opportunistic pathogen that normally inhabits the lower gastrointestinal and urogenital tracts of the human body. Approximately 10%-30 % of pregnant women have GBS vaginal sojourn.

Pregnant women are susceptible to GBS infection due to changes in the internal environment of the reproductive tract due to changes in hormone levels in the body, which will cause adverse pregnancy outcomes such as preterm labor, premature rupture of membranes, and stillbirth, and can also lead to puerperal infections in pregnant women.

Neonatal group B streptococcus is associated with perinatal infection and is an important pathogen of severe infectious diseases such as neonatal sepsis and meningitis. 40%-70% of mothers infected with GBS will transmit GBS to their newborns during delivery through the birth canal, causing serious neonatal infectious diseases such as neonatal sepsis and meningitis. If the newborns carry GBS, about 1%-3% will develop early invasive infection, of which 5% will result in death.

Features

● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 1×103Copies/mL.
● High specificity: No cross-reactivity with common pathogens of reproductive tract infections.
● Convenient: One test for multiple results.

Channel

FAM: GBS target
VIC/HEX: Internal control

PCR Amplification Conditions Setting

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 2mins No
2 1 cycle 95℃ 5mins No
3 40 cycles 95℃ 10s No
58℃ 30s Yes

Technical Parameters

Storage Liquid:≤-18℃ in dark; Lyophilization: ≤30℃ in dark    
Shelf-life 12 months
Specimen Type Genital and Rectal Secretions 
Ct ≤38
CV ≤5.0%
LoD 1×103Copies/mL
Covering Subtypes Detect group B streptococcus serotypes (I a, I b, I c, II, III, IV, V, VI, VII, VIII, IX and ND) and the results are all positive.

Specificity: Detect other genital tract and rectal swab samples such as candida albicans, trichomonas vaginalis, chlamydia trachomatis, ureaplasma urealyticum, neisseria gonorrhoeae, mycoplasma hominis, mycoplasma genitalium, herpes simplex virus, human papilloma virus , lactobacillus, gardnerella vaginalis, staphylococcus aureus, national negative reference N1-N10 (streptococcus pneumoniae, streptococcus pyogenes, streptococcus thermophilus, streptococcus mutans, streptococcus pyogenes, lactobacillus acidophilus bacillus, lactobacillus reuteri, escherichia coli DH5α, candida albicans) and human genomic DNA, the results are all negative for group B streptococcus.

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.

Main Components

Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR)

Catalogue Number Component (50 tests/kit) Specification Quantity Component Description
HWTS-UR027A GBS Reaction Buffer 1.25mL /vial 1 vial GBS gene and internal control specific primers, fluorescent probes, UNG enzyme and Taq enzyme in a certain proportion
GBS Positive Control 600μL/vial 1 vial Mixture of GBS gene recombinant plasmid and internal control recombinant plasmid
GBS Blank Control 600μL/vial 1 vial DNase/RNase free H2O

Freeze-dried Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR)

Component

Character

Catalogue Number

Component Description

HWTS-UR028A HWTS-UR028B
Specification/
Quantity
(20 tests/kit)
Specification/
Quantity
(50 tests/kit)
GBS Reaction Buffer lyophilization 1 bottle 1 bottle Contains GBS gene and internal control specific primers, fluorescent probes, UNG enzyme and Taq enzyme and prepared in a certain proportion
GBS Positive Control lyophilization 1 vial 1 vial GBS gene recombinant plasmids and internal control recombinant plasmids mix
GBS Blank Control Liquid 1 vial, 600μL/vial 1 vial, 600μL/vial DNase/RNase free H2O
Reconstituted Solution Liquid 1 vial, 1mL/vial 1 vial, 1.45mL/vial DNase/RNase free H2O, etc.

Total PCR Solution

Option 1.

Print

Option 2.

Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR)7

Reference

[1] Schrag S, Gorwitz R, Fultzbutts K, et al. Prevention of perinatal group B streptococcal disease. Revised guidelines from CDC.[J]. Pediatric Infectious Disease Journal, 2010, 59(RR-10):1-31.
[2] Yuan XY, Liu HZ, Liu JF, Sun Y, Song Y. Pathogenic mechanism, detection methods and clinical significance of group B Streptococcus. Future Microbiol. 2021 Jun; 16:671-685.
[3] Furfaro LL, Chang BJ, Payne MS. Perinatal Streptococcus agalactiae Epidemiology and Surveillance Targets. Clin Microbiol Rev. 2018 Aug 15; 31(4):e00049-18.
[4] Shi CY, Qu SH, Yang L, Yang HX. [Detection of maternal colonization of group B streptococcus in late pregnancy by real-time polymerase chain reaction and its effect on perinatal outcome]. Zhonghua Fu Chan Ke Za Zhi. 2010 Jan; 45(1):12-6. Chinese.
[5] Ferrieri P, Flores AE. Surface protein expression in group B streptococcal invasive isolates. Adv Exp Med Biol. 1997;418:635-7.


Product detail pictures:

Discount Price Urinary Reproduction - Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Discount Price Urinary Reproduction - Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Discount Price Urinary Reproduction - Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Discount Price Urinary Reproduction - Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Discount Price Urinary Reproduction - Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Discount Price Urinary Reproduction - Group B Streptococcus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures


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