Eight Kinds of Respiratory Viruses
Product name
HWTS-RT184-Eight Kinds of Respiratory Viruses Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Respiratory tract infection is the most common type of human disease, which can occur in any gender, age and region, and is one of the most important causes of morbidity and death in the world [1]. Clinically common respiratory pathogens include influenza A virus (IFV A), influenza B virus (IFV B), respiratory syncytial virus, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus (I/II/III) and mycoplasma pneumoniae, etc. [2,3]. The clinical symptoms and signs caused by respiratory tract infection are relatively similar, but the infection caused by different pathogens has different treatment methods, curative effects and course of disease [4,5]. At present, the main methods of laboratory detection of respiratory pathogens include: virus isolation, antigen detection and nucleic acid detection. This kit detects and identifies specific viral nucleic acids in individuals with signs and symptoms of respiratory infection, in conjunction with other clinical and laboratory results to assist in the diagnosis of respiratory viral infection.
Technical Parameters
| Storage |
2-8℃ |
| Shelf-life | 12 months |
| Specimen Type | Oropharyngeal swab; Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 Copies/mL |
| Specificity |
Cross reactivity: There is no cross reactivity between the kit and Boca virus, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, measles virus, human coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Lactobacillus, Corynebacterium, human genomic DNA. Interference test: Select mucin (60mg/mL), human blood (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), sodium chloride with 5% preservative (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), fluniacetone (20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L), The results showed that the interfering substances at the above concentra |
| Applicable Instruments | Applicable to type I detection reagent: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System.
Applicable to type II detection reagent: EudemonTM AIO800 (HWTS-EQ007) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Work Flow
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), and Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (which can be used with EudemonTM AIO800 (HWTS-EQ007)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
The extracted sample volume is 200μL and the recommended elution volume is 150μL.
