Enterovirus 71 Nucleic Acid
Product name
HWTS-EV022A-Enterovirus 71 Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)
HWTS-EV023A-Freeze-dried Enterovirus 71 Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)
Certificate
CE
Epidemiology
Hand-foot-and-mouth disease(HFMD) is an acute infectious disease caused by enterovirus infection. At present, a total of 108 enterovirus serotypes have been found, which are divided into four groups: A, B, C and D. The disease mostly occurs in children under 5 years old, and can cause herpes on the hands, feet, mouth and other parts, and also can cause complications such as myocarditis, pulmonary edema, aseptic meningoencephalitis of a small number of children . There are more than 20 types of enteroviruses that cause HFMD, among which enterovirus 71 (EV71) is main pathogens causing HFMD in children.Hand-foot-and-mouth disease(HFMD) is an acute infectious disease caused by enterovirus infection. At present, a total of 108 enterovirus serotypes have been found, which are divided into four groups: A, B, C and D. The disease mostly occurs in children under 5 years old, and can cause herpes on the hands, feet, mouth and other parts, and also can cause complications such as myocarditis, pulmonary edema, aseptic meningoencephalitis of a small number of children . There are more than 20 types of enteroviruses that cause HFMD, among which enterovirus 71 (EV71) is main pathogens causing HFMD in children.
Channel
FAM |
EV71 nucleic acid |
ROX |
Internal Control |
Technical Parameters
Storage | Liquid: ≤-18℃ In dark; Lyophilized: ≤30℃ In dark |
Shelf-life | Liquid: 9 months; Lyophilized: 12 months |
Specimen Type | throat swabs |
Tt | ≤28 |
CV | ≤10.0% |
LoD | 2000 Copies/mL |
Specificity | No cross-reactivity with other respiratory pathogens such as influenza A virus, influenza B virus, adenovirus, respiratory syncytial virus, Klebsiella pneumoniae and normal human throat swab samples |
Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems
SLAN ®-96P Real-Time PCR Systems LightCycler® 480 Real-Time PCR system Easy Amp Real-time Fluorescence Isothermal Detection System(HWTS1600) |
Work Flow
Option 1.
Recommended extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8)
Option 2.
Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006)