Factory Price Monkeypox Kit - Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test

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Factory Price Monkeypox Kit - Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Orthopox Virus Universal Type/Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Certificate

CE

Epidemiology

Monkeypox (MP) is an acute zoonotic infectious disease caused by Monkeypox Virus (MPV). The disease is mainly transmitted by animals, and humans could become infected by being bitten by infected animals or by direct contact with the blood, body fluids and rash of infected animals. The virus can also be transmitted between people, primarily through respiratory droplets during prolonged, direct face-to-face contact or through direct contact with a patient’s body fluids or contaminated objects[1-2].

The clinical symptoms of monkeypox infection in humans are similar to those of smallpox, generally after a 12-day incubation period, appearing fever, headache, muscle and back pain, enlarged lymph nodes, fatigue and discomfort. The rash appears after 1-3 days of the fever, usually first on the face, but also in other parts. The disease course generally lasts 2-4 weeks, and the mortality rate is 1%-10%. Lymphadenopathy is one of the main differences between this disease and smallpox[3].

Features

● Multiplex PCR Amplification Technology
● Accuracy: Internal control, fully monitor the experimental process to ensure the quality of Experiments.
● Rapid: Rapid PCR amplification shorts detection time, resulted in 40 minutes
● High sensitivity: 200 Copies/mL
● Triple PCR assay: Dual target gene(MPV-1 & MPV-2) of Monkeypox virus + human endogenous gene

Channel

Channel Monkeypox Monkeypox & Orthopox
FAM Monkeypox virus MPV-1 gene Orthopox virus universal type nucleic acid
VIC/HEX Monkeypox virus MPV-2 gene Monkeypox virus MPV-2 gene
ROX / Monkeypox virus MPV-1 gene
CY5 Internal Control Internal control

PCR Amplification Conditions Setting

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 2mins No
2 1 cycle 95℃ 1min No
3 40 cycles 95℃ 5s No
55℃ 15s Yes

Technical Parameters

Storage ≤-18℃ in dark  
Shelf-life 2 months
Specimen Type Rash Fluid, Nasopharyngeal Swab, Throat Swab, Serum 
Ct ≤38
CV ≤5.0%
LoD 200Copies/mL
Specificity
No cross-reactivity with Smallpox virus, Cowpox virus, Vaccinia virus, Herpes simplex virus, etc. 
No cross-reactivity with other pathogens that cause rash disease. 
No cross-reactivity with human genomic DNA. 
Applicable Instruments It can match the mainstream fluorescent PCR instruments on the market.
QuantStudio®5 Real-Time PCR Systems 
SLAN-96P Real-Time PCR Systems  
LightCycler®480 Real-Time PCR Systems 
LineGene 9600 Plus Real-Time PCR Detection Systems  
MA-6000 Real-Time Quantitative Thermal Cycler  
BioRad CFX96 Real-Time PCR Systems 
BioRad CFX Opus 96 Real-Time PCR Systems 

Main Components

Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Catalogue Number Component (50 tests/kit) Specification Quantity Component Description
HWTS-OT071A MPV Reaction Buffer  1.25mL /vial 1 vial Amplification reaction reagents, monkeypox virus MPV-1 and MPV-2 primer probes and internal control primer probes, etc..
MPV Positive Control  250μL/vial  1 vial Monkeypox virus and internal control mixed plasmid
MPV Blank Control 250μL/vial 1 vial DNase/RNase free H2O

Orthopox Virus Universal Type/Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Catalogue Number Component (50 tests/kit) Specification Quantity Component Description
HWTS-OT072A OPV/MPV Reaction Buffer  1.25mL /vial 1 vial Amplification reaction reagents, orthopox virus universal type and monkeypox virus primer probes, internal control primer probes, etc.
OPV/MPV Positive Control  250μL/vial  1 vial Orthopox virus universal type, monkeypox virus and internal control mixed plasmid
OPV/MPV Blank Control 250μL/vial 1 vial DNase/RNase free H2O

Total PCR Solution

Option 1.

Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)8

Option 2.

Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)9

References

[1] Centers for Disease Control and Prevention. Human monkeypox-Kasai Oriental, Zaire, 1996-1997.MMWR. 1997, 46:304-307.
[2] Beer EM, Rao VB. A systematic review of the epidemiology of human monkeypox outbreaks and implications for outbreak strategy. PLoS Negl Trop Dis 2019 10;13(10).
[3] Jezek Z, Marennikova SS, Mutumbo M, et al. Human monkeypox: a study of 2,510 contacts of 214 patients. J Infect Dis 1986 Oct;154(4).


Product detail pictures:

Factory Price Monkeypox Kit - Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Factory Price Monkeypox Kit - Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Factory Price Monkeypox Kit - Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Factory Price Monkeypox Kit - Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Factory Price Monkeypox Kit - Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures


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