Factory source Ebv Symptoms - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test

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Factory source Ebv Symptoms - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

EB Virus Nucleic Acid Detection Kit(Fluorescence PCR)

Certificate

CE

Epidemiology

EBV (Epstein-barr virus), or human herpesvirus type 4, is a common human herpesvirus[1]. In recent years, a large number of studies have proved that EBV is associated with occurrence and development of nasopharyngeal cancer, Hodgkin’s disease, T/Natural killer celllymphoma, Burkitt’s lymphoma, breast cancer, gastric cancer and other malignant tumors. And it is also closely associated with post-transplantlymphoproliferative disorders, post-transplant smooth muscle tumor and acquired immunedeficiency syndrome(AIDS) related lymphoma, multiple sclerosis, primary central nervous system lymphoma or leiomyosarcoma[2-4].

Features

● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 500Copies/mL.
● Convenient: One test for multiple results.

Channel

FAM: EBV
VIC (HEX): Internal control

PCR Amplification Conditions Setting

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 2mins No
2 1 cycle 95℃ 5mins No
3 40 cycles 95℃ 15s No
58℃ 30s Yes

Technical Parameters

Storage ≤-18℃ In dark
Shelf-life 12 months
Specimen Type Whole blood, Plasma, Serum
Ct ≤38
CV ≤5.0%
LoD 500Copies/mL

Specificity: It has no cross-reactivity with other pathogens (such as human herpesvirus 1, 2, 3, 6, 7, 8, hepatitis B virus, cytomegalovirus, influenza A, etc.) or bacteria (Staphylococcus aureus, Candida albicans, etc.)

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.

Main Components

Catalogue Number Component (50 tests/kit) Specification Quantity Component Description
HWTS-OT061A EB Reaction Buffer 1.175mL/vial 1 vial Amplification reaction reagents, EB virus primer probes, internal reference primer probes, etc.
EB Enzyme Mix 75μL/vial 1 vial Taq enzyme, UDG enzyme, etc.
EB Positive Control 600μL/vial 1 vial Mixed solution of viral target gene and internal reference
EB Blank Control 600μL/vial 1 vial DNase/RNase free H2O

Total PCR Solution

EB Virus Nucleic Acid Detection Kit(Fluorescence PCR)6

Reference

[1] ohen JI, Jaffe ES, Dale JK, et al. Characterization and treatment of chronic active Epstein-Barr virus disease: a 28-year experience in the United States[J]. Blood, 2011, 117(22): 5835-5849.
[2] Jahangiryan A, Kheirandish M, Samiee S, et al. Determination of Epstein-Barr virus (EBV) incidence in umbilical cord blood (UCB) and assessment of virus DNA via Real-time PCR[J]. Annals of Cancer Research and Therapy, 2021, 29(1):114-120.
[3] Petersson F. Nasopharyngeal carcinoma:a review[J].Semin Diagn Pathol, 2015, 32(1):54-73. [4]Chen J N, He D, Tang F, et al. Epstein-Barr virus-associated gastric carcinoma:a newly defined entity[J]. J Clin Gastroenterol, 2012, 46(4):262-271.


Product detail pictures:

Factory source Ebv Symptoms - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Factory source Ebv Symptoms - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Factory source Ebv Symptoms - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Factory source Ebv Symptoms - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures

Factory source Ebv Symptoms - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test detail pictures


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