Factory source Ebv Symptoms - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test
Factory source Ebv Symptoms - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test Detail:
Product name
EB Virus Nucleic Acid Detection Kit(Fluorescence PCR)
Certificate
CE
Epidemiology
EBV (Epstein-barr virus), or human herpesvirus type 4, is a common human herpesvirus[1]. In recent years, a large number of studies have proved that EBV is associated with occurrence and development of nasopharyngeal cancer, Hodgkin’s disease, T/Natural killer celllymphoma, Burkitt’s lymphoma, breast cancer, gastric cancer and other malignant tumors. And it is also closely associated with post-transplantlymphoproliferative disorders, post-transplant smooth muscle tumor and acquired immunedeficiency syndrome(AIDS) related lymphoma, multiple sclerosis, primary central nervous system lymphoma or leiomyosarcoma[2-4].
Features
● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 500Copies/mL.
● Convenient: One test for multiple results.
Channel
FAM: EBV
VIC (HEX): Internal control
PCR Amplification Conditions Setting
Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
1 | 1 cycle | 50℃ | 2mins | No |
2 | 1 cycle | 95℃ | 5mins | No |
3 | 40 cycles | 95℃ | 15s | No |
58℃ | 30s | Yes |
Technical Parameters
Storage | ≤-18℃ In dark |
Shelf-life | 12 months |
Specimen Type | Whole blood, Plasma, Serum |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 500Copies/mL |
Specificity: It has no cross-reactivity with other pathogens (such as human herpesvirus 1, 2, 3, 6, 7, 8, hepatitis B virus, cytomegalovirus, influenza A, etc.) or bacteria (Staphylococcus aureus, Candida albicans, etc.)
Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.
Main Components
Catalogue Number | Component (50 tests/kit) | Specification | Quantity | Component Description |
HWTS-OT061A | EB Reaction Buffer | 1.175mL/vial | 1 vial | Amplification reaction reagents, EB virus primer probes, internal reference primer probes, etc. |
EB Enzyme Mix | 75μL/vial | 1 vial | Taq enzyme, UDG enzyme, etc. | |
EB Positive Control | 600μL/vial | 1 vial | Mixed solution of viral target gene and internal reference | |
EB Blank Control | 600μL/vial | 1 vial | DNase/RNase free H2O |
Total PCR Solution
Reference
[1] ohen JI, Jaffe ES, Dale JK, et al. Characterization and treatment of chronic active Epstein-Barr virus disease: a 28-year experience in the United States[J]. Blood, 2011, 117(22): 5835-5849.
[2] Jahangiryan A, Kheirandish M, Samiee S, et al. Determination of Epstein-Barr virus (EBV) incidence in umbilical cord blood (UCB) and assessment of virus DNA via Real-time PCR[J]. Annals of Cancer Research and Therapy, 2021, 29(1):114-120.
[3] Petersson F. Nasopharyngeal carcinoma:a review[J].Semin Diagn Pathol, 2015, 32(1):54-73. [4]Chen J N, He D, Tang F, et al. Epstein-Barr virus-associated gastric carcinoma:a newly defined entity[J]. J Clin Gastroenterol, 2012, 46(4):262-271.
Product detail pictures:
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Our pursuit and company goal is to Always satisfy our customer requirements. We continue to develop and design superior quality products for both our old and new customers and achieve a win-win prospect for our clients as well as us for Factory source Ebv Symptoms - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test , The product will supply to all over the world, such as: Albania, Sudan, Riyadh, We welcome you to visit our company and factory. It is also convenient to visit our website. Our sales team will offer you the best service. If you need more information, please feel free to contact us by E-mail or telephone. We are sincerely hope to establish a good long-term business relationship with you through this opportunity, based on equal, mutual benefit from now till the future.
By Agustin from Bhutan - 2018.06.18 19:26
It can be said that this is a best producer we encountered in China in this industry, we feel lucky to work with so excellent manufacturer.
By David Eagleson from Lithuania - 2018.12.30 10:21