This kit is suitable for in vitro qualitative detection of 28 types of human papilloma viruses (HPV) (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) nucleic acid in male/female urine and female cervical exfoliated cells. HPV 16/18 can be typed, the remaining types cannot be completely typed, providing an auxiliary means for the diagnosis and treatment of HPV infection.