This kit is used for qualitative and genotyping detection of nucleic acid of 28 types of human papillomavirus (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) in male/female urine and female cervical exfoliated cells, providing auxiliary means for the diagnosis and treatment of HPV infection.