Freeze-dried Chlamydia Trachomatis
Product name
HWTS-UR032C/D-Freeze-dried Chlamydia Trachomatis Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)
Epidemiology
Chlamydia trachomatis (CT) is a kind of prokaryotic microorganism that is strictly parasitic in eukaryotic cells[1]. Chlamydia trachomatis is divided into A-K serotypes according to the serotype method. Urogenital tract infections are mostly caused by trachoma biological variant D-K serotypes, and males are mostly manifested as urethritis, which can be relieved without treatment, but most of them become chronic, periodically aggravated, and can be combined with epididymitis, proctitis, etc[2]. Females can be caused with urethritis, cervicitis, etc., and more serious complications of salpingitis[3].
Channel
| FAM | Chlamydia trachomatis (CT) |
| ROX |
Internal Control |
Technical Parameters
| Storage |
≤30℃ |
| Shelf-life | 12 months |
| Specimen Type | Female cervical swab
Male urethral swab Male urine |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | 400 Copies/mL |
| Specificity | there is no cross-reactivity between this kit and other genitourinary tract infection pathogens such as high-risk Human papillomavirus type 16, Human papillomavirus type 18, Herpes simplex virus type Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylococcus epidermidis, Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei and human genomic DNA, etc. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System and BioRad CFX Opus 96 Real-Time PCR System Easy Amp Real-time Fluorescence Isothermal Detection System（HWTS-1600）. |
Work Flow
Option 1.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). The extraction should be carried out in strict accordance with the IFU. Add the sample DNA extracted by the sample release reagent into the reaction buffer and test on the instrument directly, or the extracted samples should be stored at 2-8℃ for no more than 24 hours.
Option 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). The extraction should be carried out in strict accordance with the IFU, and the recommended elution volume is 80μL. The sample DNA extracted by the magnetic bead method is heated at 95°C for 3 minutes and then immediately ice-bathed for 2 minutes. Add the processed sample DNA into the reaction buffer and test on the instrument or the processed samples should be store below -18°C for no more than 4 months. The number of repeated freezing and thawing should not exceed 4 cycles.
