Hepatitis A Virus
Product name
HWTS-HP005 Hepatitis A Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Hepatitis A virus (HAV) is the main cause of acute viral hepatitis. The virus is a positive-sense single-stranded RNA virus and belongs to the Hepadnavirus genus of the family Picornaviridae. Hepatitis A virus, mainly transmitted by the fecal-oral route, resistant to heat, acids, and most organic solvents, can survive for a long time in shellfish, water, soil, or seabed sediments[1-3]. It is transmitted by having contaminated food or water, or directly it transmitted from person-to-person. Foods associated with HAV include oysters and clams, strawberries, raspberries, blueberries, dates, green leafy vegetables, and semi-dried tomatoes[4‒6].
Channel
| FAM | HAV nucleic acid |
| ROX |
Internal Control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | Liquid: 9 months, Lyophilized: 12 months |
| Specimen Type | Serum/stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 2 Copies/μL |
| Specificity | Use the kits to test other hepatitis viruses such as hepatitis B, C, D, E, enterovirus 71, coxsackie virus, Epstein-Barr virus, norovirus, HIV and human genome.There is no cross-reactivity. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Serum samples
Option 1.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). It should be extracted according to the instructions. The recommended elution volume is 80µL.
Option 2.
TIANamp Virus DNA/RNA Kit (YDP315-R) manufactured by Tiangen Biotech (Beijing) Co., Ltd. It should be extracted according to the instructions. The extracted sample volume is 140μL. The recommended elution volume is 60µL.
2.Stool Samples
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). It should be extracted according to the instructions. The recommended elution volume is 80µL.
