Hepatitis B Virus
Product Name
HWTS-HP001-Hepatitis B Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Hepatitis B is an infectious disease with liver and multiple organ lesion caused by the hepatitis B virus (HBV). Most people experience symptoms such as extreme fatigue, appetite loss, lower limbs or whole-body edema, hepatomegaly, etc. 5% of adult patients and 95% of children patients infected from their mother cannot clean the HBV virus efficiently in persistent infection and progress to liver cirrhosis or primary liver cell carcinoma.
Channel
| FAM | HBV-DNA |
| VIC (HEX) | Internal reference |
Technical Parameters
| Storage | ≤-18℃ In dark |
| Shelf-life | 12 months |
| Specimen Type | Venous blood |
| Ct | ≤33 |
| CV | ≤5.0％ |
| LoD | 25IU/mL |
|
Specificity |
There is no cross-reactivity with Cytomegalovirus, EB virus, HIV, HAV, Syphilis, Human Herpesvirus-6, HSV-1/2, Influenza A, Propionibacterium Acnes, Staphylococcus Aureus and Candida albican |
| Applicable Instruments | It can match the mainstream fluorescent PCR instruments on the market.
ABI 7500 Real-Time PCR Systems ABI 7500 Fast Real-Time PCR Systems SLAN-96P Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Recommended extraction reagents: Macro & Micro-Test Virus DNA/RNA Kit (HWTS-3017) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. The extraction should be started according to the IFU of the extraction reagent. The extracted sample volume is 200µL and the recommended elution volume is 80 μL.
Recommended extraction reagents: Nucleic Acid Extraction or Purification Reagents (YDP315). The extraction should be started in strict accordance with the IFU. The extracted sample volume is 200µL and the recommended elution volume is 100 μL.
