Hepatitis E Virus
Product name
HWTS-HP006 Hepatitis E Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Hepatitis E virus (HEV) is an RNA virus that causes global health problems. It has a wide host range and has the property of crossing interspecies barriers. It is one of the most important zoonotic pathogens and cause great harm to human and animal. HEV is mainly transmitted through fecal-oral transmission, and can also be transmitted vertically through embryos or blood. Among them, in the fecal-oral transmission route, HEV-contaminated water and food spread widely, and the risk of HEV infection in humans and animals is high[1-2].
Channel
| FAM | HEV nucleic acid |
| ROX |
Internal Control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Throat swab |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Copies/μL |
| Specificity |
Hepatitis E virus (HEV) is an RNA virus that causes global health problems. It has a wide host range and has the property of crossing interspecies barriers. It is one of the most important zoonotic pathogens and cause great harm to human and animal. HEV is mainly transmitted through fecal-oral transmission, and can also be transmitted vertically through embryos or blood. Among them, in the fecal-oral transmission route, HEV-contaminated water and food spread widely, and the risk of HEV infection in humans and animals is high[1-2]. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System
Applied Biosystems 7500 Fast Real-Time PCR System QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). It should be extracted according to the instructions. The recommended elution volume is 80µL.
Option 2
TIANamp Virus DNA/RNA Kit (YDP315-R) manufactured by Tiangen Biotech (Beijing) Co., Ltd. It should be extracted strictly according to the instructions. The extracted sample volume is 140μL. The recommended elution volume is 60µL.v
