Herpes Simplex Virus Type 1
Product name
HWTS-UR006 Herpes Simplex Virus Type 1 Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Sexually transmitted diseases (STDs) are still one of the important threats to global public health security, which can lead to infertility, premature delivery, tumors and various serious complications[3-6]. There are many kinds of STD pathogens, including bacteria, viruses, chlamydia, mycoplasma and spirochetes. Common species include neisseria gonorrhoeae, mycoplasma genitalium, chlamydia trachomatis, herpes simplex virus type 1, herpes simplex virus type 2, mycoplasma hominis, ureaplasma urealyticum, etc.
Channel
| FAM | Herpes Simplex Virus Type 1 (HSV1) |
| ROX |
Internal Control |
Technical Parameters
| Storage |
-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Female cervical swab，Male urethral swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Copies/mL |
| Specificity | Test other STD infection pathogens, such as treponema pallidum, chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma hominis, mycoplasma genitalium, ureaplasma urealyticum, etc., there is no cross-reactivity. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
Macro & Micro-Test Sample Release Reagent(HWTS-3005-8), the extraction should be conducted according to the IFU strictly.
Option 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). The extraction should be carried out according to the IFU, and the recommended elution volume is 80μL.
Option 3.
Nucleic Acid Extraction or Purification Reagent (YDP302) by Tiangen Biotech (Beijing) Co., Ltd. The extraction should be carried out in strict accordance with the IFU, and the recommended elution volume is 80μL.
The extracted DNA samples should be tested immediately or stored below -18°C for no more than 7 months. The number of repeated freezing and thawing should not exceed 4 cycles.
