Herpes Simplex Virus Type 1/2,(HSV1/2) Nucleic Acid
Product name
HWTS-UR018A-Herpes simplex virus type 1/2, (HSV1/2) nucleic acid detection kit (Fluorescence PCR)
Epidemiology
Sexually transmitted diseases (STD) are still one of the major threats to global public health security. Such diseases can lead to infertility, premature fetal delivery, tumor and various serious complications. There are many types of STD pathogens, including bacteria, viruses, chlamydia, mycoplasma and spirochetes, among which Neisseria gonorrhoeae, Mycoplasma genitalium, Chlamydia trachomatis, HSV1, HSV2, Mycoplasma hominis, and Ureaplasma urealyticum are common.
Genital herpes is a common sexually transmitted disease caused by HSV2, which is highly infectious. In recent years, the incidence of genital herpes has increased significantly, and due to an increase in risky sexual behaviors, the detection rate for HSV1 in genital herpes has increased and was reported to be as high as 20%-30%. An initial infection with the genital herpes virus is mostly silent without obvious clinical symptoms except local herpes in the mucosa or skin of a few patients. Since genital herpes is characterized by lifelong viral shedding and proneness towards recurrence, it is important to screen the pathogens as soon as possible and block its transmission.
Channel
FAM | HSV1 |
CY5 | HSV2 |
VIC(HEX) | Internal Control |
Technical Parameters
Storage | Liquid: ≤-18℃ In dark |
Shelf-life | 12 months |
Specimen Type | urethral secretions, cervical secretions |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 Copies/reaction |
Specificity | There is no cross-reactivity with other STD pathogens such as Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum. |
Applicable Instruments | It can match the mainstream fluorescent PCR instruments on the market.
Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Recommended extraction reagent: Macro & Micro-Test Sample Release Reagent(HWTS-3005-8). The extraction should be strictly conducted according to the instructions.
Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The extraction should be strictly conducted according to the instructions. The recommended elution volume should be 80 μL.
Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP315) by Tiangen Biotech(Beijing) Co.,Ltd. The extraction should be strictly conducted according to the instructions. The recommended elution volume should be 80 μL.