Herpes Simplex Virus Type 2 Nucleic Acid
Product Name
HWTS-UR007A-Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Fluorescence PCR)
Intended Use
This kit is used for qualitative detection of herpes simplex virus type 2 nucleic acid in male urethral swab and female cervical swab samples.
Epidemiology
Herpes Simplex Virus Type 2 (HSV2) is a circular virus synthesized with tegument, capsid, core, and envelope, and contains double-stranded linear DNA. Herpes virus can enter the body through direct contact or sexual contact with skin and mucous membranes, and is divided into primary and recurrent. Reproductive tract infection is mainly caused by HSV2, male patients are manifested as penile ulcers, and female patients are manifested as cervical, vulvar, and vaginal ulcers. The initial infections of genital herpes virus are mostly recessive infections, except for a few local herpes with mucous membranes or skin, most of which have no obvious clinical symptoms. Genital herpes infection has the characteristics of lifelong virus carrying and easy recurrence, and both patients and carriers are the source of infection of the disease. In China, the serological positive rate of HSV2 is about 10.80% to 23.56%. The stage of HSV2 infection can be divided into primary infection and recurrent infection, and about 60% of HSV2 infected patients relapse.
Epidemiology
FAM: Herpes Simplex Virus Type 2 (HSV2)·
VIC(HEX): Internal Control
PCR Amplification Conditions Setting
Step |
Cycles |
Temperature |
Time |
Collect Fluorescent Signals or Not |
1 |
1 Cycle |
50℃ |
5mins |
No |
2 |
1 Cycle |
95℃ |
10mins |
No |
3 |
40 Cycles |
95℃ |
15secs |
No |
4 |
58℃ |
31secs |
Yes |
Technical Parameters
Storage | |
Liquid |
≤-18℃ In dark |
Shelf-life | 12 months |
Specimen Type |
Female cervical swab, Male urethral swab |
Ct |
≤38 |
CV |
≤5.0% |
LoD | 50Copies/reaction |
Specificity |
There is no cross-reactivity with other STD pathogens, such as Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium and etc. |
Applicable Instruments |
It can match the mainstream fluorescent PCR instruments on the market. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System. |