High Quality for Hpv Genotyping Pcr - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test

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High Quality for Hpv Genotyping Pcr - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test Detail:

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28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR )

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Epidemiology

Cervical cancer is one of the most common malignant tumors in the female reproductive tract. The studies have shown that persistent infection and multiple infections of human papillomavirus is one of the important cause of cervical cancer. At present, there is still a lack of recognized effective treatment methods for HPV. Therefore, early detection and early prevention of cervical HPV is the key to blocking canceration. The establishment of a simple, specific and rapid pathogenic diagnostic method is of great significance in the clinical diagnosis of cervical cancer.

Features

● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 25 Copies/reaction
● High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.
● Convenient: One test for multiple results.

Channel

S/N	Channel	Type
PCR-Mix1	FAM	16, 18, 31, 56
	VIC(HEX)	Internal Control
	CY5	45, 51, 52, 53
	ROX	33, 35, 58, 66
PCR-Mix2	FAM	6, 11, 54, 83
	VIC(HEX)	26, 44, 61, 81
	CY5	40, 42, 43, 82
	ROX	39, 59, 68, 73

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	2 mins	No
2	1 cycle	95℃	10 mins	No
3	10 cycle	95℃	15 s	No
3	10 cycle	62℃	20 s	No
4	30 cycle	95℃	15 s	No
4	30 cycle	58℃	30 s	Yes

Technical Parameters

Storage	≤-18℃ in dark
Shelf-life	12 months
Specimen Type	Cervical exfoliated cells
Ct	≤28
CV	≤5.0％
LoD	25 Copies/reaction
Specificity	No cross-reactivity with common reproductive tract pathogens (such as ureaplasma urealyticum, genital tract chlamydia trachomatis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella and other HPV types not covered in the kit, etc).
Applicable Instruments	It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems
ABI 7500 Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems
LightCycler®480 Real-Time PCR Systems
LineGene 9600 Plus Real-Time PCR Detection Systems
MA-6000 Real-Time Quantitative Thermal Cycler

Main Components

Catalogue Number	Component (50 tests/kit)	Specification	Quantity	Component Description
HWTS-CC003A	HPV WELL1	1.3mL/vial	1 vial	Mixture of specific primers and probes of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66
	HPV WELL2	1.3mL/vial	1 vial	Mixture of specific primers and probes of HPV6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83
	HPV Enzyme Mix	55uL/vial	1 vial	Mixture of Taq DNA polymerase, Uracil-DNA Glycosylase and glycerin
	HPV Positive Control WELL1	150μL/vial	1 vial	Mixture of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66 templates and Internal Control template
	HPV Positive Control WELL2	150μL/vial	1 vial	Mixture of 6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83 templates
	HPV Blank Control	300μL/vial	1 vial	RNase/DNase free H₂O

Total PCR Solution

Option 1.

Fluorescence PCR3

Option 2.

Fluorescence PCR4

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