High Quality for Hpv Genotyping Pcr - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test
High Quality for Hpv Genotyping Pcr - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test Detail:
Product name
28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR )
Certificate
CE
Epidemiology
Cervical cancer is one of the most common malignant tumors in the female reproductive tract. The studies have shown that persistent infection and multiple infections of human papillomavirus is one of the important cause of cervical cancer. At present, there is still a lack of recognized effective treatment methods for HPV. Therefore, early detection and early prevention of cervical HPV is the key to blocking canceration. The establishment of a simple, specific and rapid pathogenic diagnostic method is of great significance in the clinical diagnosis of cervical cancer.
Features
● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 25 Copies/reaction
● High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.
● Convenient: One test for multiple results.
Channel
S/N | Channel | Type |
PCR-Mix1 | FAM | 16, 18, 31, 56 |
VIC(HEX) | Internal Control | |
CY5 | 45, 51, 52, 53 | |
ROX | 33, 35, 58, 66 | |
PCR-Mix2 | FAM | 6, 11, 54, 83 |
VIC(HEX) | 26, 44, 61, 81 | |
CY5 | 40, 42, 43, 82 | |
ROX | 39, 59, 68, 73 |
PCR Amplification Conditions Setting
Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
1 | 1 cycle | 50℃ | 2 mins | No |
2 | 1 cycle | 95℃ | 10 mins | No |
3 | 10 cycle | 95℃ | 15 s | No |
62℃ | 20 s | No | ||
4 | 30 cycle | 95℃ | 15 s | No |
58℃ | 30 s | Yes |
Technical Parameters
Storage | ≤-18℃ in dark |
Shelf-life | 12 months |
Specimen Type | Cervical exfoliated cells |
Ct | ≤28 |
CV | ≤5.0% |
LoD | 25 Copies/reaction |
Specificity | No cross-reactivity with common reproductive tract pathogens (such as ureaplasma urealyticum, genital tract chlamydia trachomatis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella and other HPV types not covered in the kit, etc). |
Applicable Instruments | It can match the mainstream fluorescent PCR instruments on the market. |
SLAN-96P Real-Time PCR Systems | |
ABI 7500 Real-Time PCR Systems | |
QuantStudio®5 Real-Time PCR Systems | |
LightCycler®480 Real-Time PCR Systems | |
LineGene 9600 Plus Real-Time PCR Detection Systems | |
MA-6000 Real-Time Quantitative Thermal Cycler |
Main Components
Catalogue Number | Component (50 tests/kit) | Specification | Quantity | Component Description |
HWTS-CC003A | HPV WELL1 | 1.3mL/vial | 1 vial | Mixture of specific primers and probes of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66 |
HPV WELL2 | 1.3mL/vial | 1 vial | Mixture of specific primers and probes of HPV6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83 | |
HPV Enzyme Mix | 55uL/vial | 1 vial | Mixture of Taq DNA polymerase, Uracil-DNA Glycosylase and glycerin | |
HPV Positive Control WELL1 | 150μL/vial | 1 vial | Mixture of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66 templates and Internal Control template | |
HPV Positive Control WELL2 | 150μL/vial | 1 vial | Mixture of 6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83 templates | |
HPV Blank Control | 300μL/vial | 1 vial | RNase/DNase free H2O |
Total PCR Solution
Option 1.
Option 2.
Product detail pictures:
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Attaining consumer satisfaction is our firm's purpose for good. We'll make wonderful efforts to produce new and top-quality merchandise, meet up with your special necessities and supply you with pre-sale, on-sale and after-sale products and services for High Quality for Hpv Genotyping Pcr - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test , The product will supply to all over the world, such as: Sierra Leone, kazakhstan, Swiss, We are trying our best to make more customers happy and satisfied. we sincerely hope to establish a good long-term business relationship with your esteemed company thought this opportunity,based on equal, mutual beneficial and win win business from now till the future.
By Sahid Ruvalcaba from Ecuador - 2018.02.12 14:52
The factory has advanced equipment, experienced staffs and good management level, so product quality had assurance, this cooperation is very relaxed and happy!
By Hilda from Chile - 2017.12.19 11:10