High reputation Hpv 16 - 14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR) – Macro & Micro-Test

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High reputation Hpv 16 - 14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR)
Freeze-dried 14 Types of High-risk Human Papilloma Virus (16/18 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)

Certificate

CE

Epidemiology

Cervical cancer is one of the most common malignant tumors in the female reproductive tract. It has been shown that persistent HPV infection and multiple infections are one of major causes of cervical cancer. Currently there is still a lack of generally accepted effective treatments for cervical cancer caused by HPV. Therefore, early detection and prevention of cervical infection caused by HPV are the keys to prevention of cervical cancerization. Establishment of simple, specific and rapid diagnostic tests for pathogens is of great significance for clinical diagnosis of cervical cancer.

Features

● Multiplex PCR Amplification Technology
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 25 Copies/reaction
● High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.
● Convenient: One test for multiple results

Channel

Channel Type
FAM HPV 18
VIC/HEX HPV 16
ROX HPV 31, 33, 35, 39, 45,51,52, 56, 58, 59, 66, 68
CY5 Internal Control

PCR Amplification Conditions Setting

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 2mins No
2 1 cycle 95℃ 10mins No
3 10 cycles 95℃ 15 s No
62℃ 20 s No
4 30 cycles 95℃ 15 s No
58℃ 30 s Yes

Technical Parameters

Storage ≤-18℃ in dark  
Shelf-life 12 months
Specimen Type Cervical exfoliated cells 
Ct ≤28
CV ≤5.0%
LoD Liquid
Specificity No cross-reactivity with common reproductive tract pathogens (such as ureaplasma urealyticum, genital tract chlamydia trachomatis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella and other HPV types not covered in the kit, etc).
Applicable Instruments It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems  
ABI 7500 Real-Time PCR Systems 
QuantStudio®5 Real-Time PCR Systems 
LightCycler®480 Real-Time PCR Systems 
LineGene 9600 Plus Real-Time PCR Detection Systems  
MA-6000 Real-Time Quantitative Thermal Cycle 

Main Components

14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR)

Catalogue Number Component (50 tests/kit) Specification Quantity Component Description
HWTS-CC007A HPV Reaction Buffer 950μL/vial 1 vial Amplification reagents
HPV Primer and Probe Mix 300μL/vial 1 vial Mix of various types of primers and probes
HPV Positive Control 600μL/vial 1 vial Mix of HPV 16, 18, 33 and internal control Templates
HPV Blank Control 600μL/vial 1 vial DNase/RNase free H2O

Freeze-dried 14 Types of High-risk Human Papilloma Virus (16/18 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)

Component Character Catalogue Number Component Description
HWTS-CC010A HWTS-CC010B
Specification/Quantity
(20 tests/kit)
Specification/Quantity
(50 tests/kit)
HPV Reaction Buffer Lyophilization 1 bottle 1 bottle Amplification reaction reagents and kinds of primers, probes
HPV Positive Control Lyophilization 1 vial 1 vial Mixture of HPV16, 18, 33 and their internal control plasmids
HPV Blank Control Liquid 1 vial, 600μL/vial 1 vial, 600μL/vial DNase/RNase free H2O
Reconstituted Solution Liquid 1 vial, 1mL/vial 1 vial, 1.45mL/vial DNase/RNase free H2O, etc.

Total PCR Solution

Option 1.

14 High-Risk HPV with 16 18-6

Option 2.

14 High-Risk HPV with 16 18-7

References

1. Guo Yumei, Sun Jie, Chen Yadan, et al. HPV testing and its significance in the diagnosis of cervical cancer [J]. Chinese Journal of Laboratory Diagnosis, 2009,13: 1306-1308.
2. Li Hua, Gao Guolan. Advance in research on HPV and cervical cancer [J]. The Practical Journal of Cancer, 2007, 22: 420-422.
3. He Guishan. Advance in research on HPV detection methods [J]. Gansu Medical Journal, 2009, 28: 182-183.
4. Ning Tang, Shihai Huang, Brian Erickson, et al. High-risk HPV detection and concurrent HPV 16 and 18 typing with abbott realtime high risk HPV test [J]. Journal of Clinical Virology, 2009, 45: S25-S28.


Product detail pictures:

High reputation Hpv 16 - 14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

High reputation Hpv 16 - 14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

High reputation Hpv 16 - 14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

High reputation Hpv 16 - 14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

High reputation Hpv 16 - 14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures


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  • We have been appreciated the Chinese manufacturing, this time also did not let us disappoint,good job!
    5 Stars By Miranda from Mecca - 2017.10.23 10:29
    High Quality, High Efficiency, Creative and Integrity, worth having long-term cooperation! Looking forward to the future cooperation!
    5 Stars By ron gravatt from Oman - 2017.03.08 14:45
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