High reputation Hpv 16 - 14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR) – Macro & Micro-Test

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High reputation Hpv 16 - 14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

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14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR)
Freeze-dried 14 Types of High-risk Human Papilloma Virus (16/18 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)

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Epidemiology

Cervical cancer is one of the most common malignant tumors in the female reproductive tract. It has been shown that persistent HPV infection and multiple infections are one of major causes of cervical cancer. Currently there is still a lack of generally accepted effective treatments for cervical cancer caused by HPV. Therefore, early detection and prevention of cervical infection caused by HPV are the keys to prevention of cervical cancerization. Establishment of simple, specific and rapid diagnostic tests for pathogens is of great significance for clinical diagnosis of cervical cancer.

Features

● Multiplex PCR Amplification Technology
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 25 Copies/reaction
● High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.
● Convenient: One test for multiple results

Channel

Channel	Type
FAM	HPV 18
VIC/HEX	HPV 16
ROX	HPV 31, 33, 35, 39, 45,51,52, 56, 58, 59, 66, 68
CY5	Internal Control

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	2mins	No
2	1 cycle	95℃	10mins	No
3	10 cycles	95℃	15 s	No
3	10 cycles	62℃	20 s	No
4	30 cycles	95℃	15 s	No
4	30 cycles	58℃	30 s	Yes

Technical Parameters

Storage	≤-18℃ in dark
Shelf-life	12 months
Specimen Type	Cervical exfoliated cells
Ct	≤28
CV	≤5.0％
LoD	Liquid
Specificity	No cross-reactivity with common reproductive tract pathogens (such as ureaplasma urealyticum, genital tract chlamydia trachomatis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella and other HPV types not covered in the kit, etc).
Applicable Instruments	It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems
ABI 7500 Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems
LightCycler®480 Real-Time PCR Systems
LineGene 9600 Plus Real-Time PCR Detection Systems
MA-6000 Real-Time Quantitative Thermal Cycle

Main Components

14 High-Risk HPV with 16/18 Genotyping Test Kit (Fluorescence PCR)

Catalogue Number	Component (50 tests/kit)	Specification	Quantity	Component Description
HWTS-CC007A	HPV Reaction Buffer	950μL/vial	1 vial	Amplification reagents
	HPV Primer and Probe Mix	300μL/vial	1 vial	Mix of various types of primers and probes
	HPV Positive Control	600μL/vial	1 vial	Mix of HPV 16, 18, 33 and internal control Templates
	HPV Blank Control	600μL/vial	1 vial	DNase/RNase free H₂O

Freeze-dried 14 Types of High-risk Human Papilloma Virus (16/18 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)

Component	Character	Catalogue Number		Component Description
		HWTS-CC010A	HWTS-CC010B
		Specification/Quantity (20 tests/kit)	Specification/Quantity (50 tests/kit)
HPV Reaction Buffer	Lyophilization	1 bottle	1 bottle	Amplification reaction reagents and kinds of primers, probes
HPV Positive Control	Lyophilization	1 vial	1 vial	Mixture of HPV16, 18, 33 and their internal control plasmids
HPV Blank Control	Liquid	1 vial, 600μL/vial	1 vial, 600μL/vial	DNase/RNase free H₂O
Reconstituted Solution	Liquid	1 vial, 1mL/vial	1 vial, 1.45mL/vial	DNase/RNase free H₂O, etc.

Total PCR Solution

Option 1.

14 High-Risk HPV with 16 18-6

Option 2.

14 High-Risk HPV with 16 18-7

References

1. Guo Yumei, Sun Jie, Chen Yadan, et al. HPV testing and its significance in the diagnosis of cervical cancer [J]. Chinese Journal of Laboratory Diagnosis, 2009,13: 1306-1308.
2. Li Hua, Gao Guolan. Advance in research on HPV and cervical cancer [J]. The Practical Journal of Cancer, 2007, 22: 420-422.
3. He Guishan. Advance in research on HPV detection methods [J]. Gansu Medical Journal, 2009, 28: 182-183.
4. Ning Tang, Shihai Huang, Brian Erickson, et al. High-risk HPV detection and concurrent HPV 16 and 18 typing with abbott realtime high risk HPV test [J]. Journal of Clinical Virology, 2009, 45: S25-S28.

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