Human CYP2C19 Gene Polymorphism

Short Description:

This kit is used for in vitro qualitative detection of the polymorphism of CYP2C19 genes CYP2C19*2 (rs4244285, c.681G>A), CYP2C19*3 (rs4986893, c.636G>A), CYP2C19*17 (rs12248560, c.806>T) in genomic DNA of human whole blood samples.


Product Detail

Product Tags

Product name

HWTS-GE012A-Human CYP2C19 Gene Polymorphism Detection Kit (Fluorescence PCR)

Certificate

CE/TFDA

Epidemiology

CYP2C19 is one of the important drug metabolizing enzymes in the CYP450 family. Many endogenous substrates and about 2% of clinical drugs are metabolized by CYP2C19, such as the metabolism of antiplatelet aggregation inhibitors (like clopidogrel), proton pump inhibitors (omeprazole), anticonvulsants, etc. CYP2C19 gene polymorphisms also have differences in the metabolizing ability of related drugs. These point mutations of *2 (rs4244285) and *3 (rs4986893) cause the loss of the enzyme activity encoded by the CYP2C19 gene and the weakness of metabolic substrate ability, and increase the blood concentration, so as to cause adverse drug reactions related to blood concentration. *17 (rs12248560) could increase the enzyme activity encoded by CYP2C19 gene, the production of active metabolites, and enhance the platelet aggregation inhibition and increase the bleeding risk. For people with slow metabolism of drugs, taking normal doses for a long time will cause serious toxic and side effects: mainly liver damage, hematopoietic system damage, central nervous system damage, etc., that can lead to death in severe cases. According to the individual differences in the corresponding drug metabolism, it is generally divided into four phenotypes, namely ultra-fast metabolism (UM,*17/*17,*1/*17), fast metabolism (RM,*1/*1) , intermediate metabolism (IM, *1/*2, *1/*3), slow metabolism (PM, *2/*2, *2/*3, *3/*3).

Channel

FAM CYP2C19*2
CY5 CYP2C9*3
ROX CYP2C19*17
VIC/HEX IC

Technical Parameters

Storage Liquid: ≤-18℃
Shelf-life 12 months
Specimen Type Fresh EDTA anticoagulated blood
CV ≤5.0%
LoD 1.0ng/μL
Specificity There is no cross-reactivity with other highly consistent sequences (CYP2C9 gene) in the human genome. The mutations of CYP2C19*23, CYP2C19*24 and CYP2C19*25 sites outside the detection range of this kit have no effect on the detection effect of this kit.
Applicable Instruments Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Recommended extraction reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The extraction should be extracted according to the instructions. The extraction sample volume is 200μL , and the recommended elution volume is 100μL.

Recommended extraction reagent: Wizard® Genomic DNA Purification Kit (Catalog No.: A1120) by Promega, Nucleic Acid Extraction or Purification Reagent(YDP348) by Tiangen Biotech(Beijing) Co.,Ltd. should be extracted according to the extraction instructions, and the recommended extraction volume is 200 μL and the recommended elution volume is 160 μL.


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