Human Papillomavirus (28 Types) Genotyping
Product name
HWTS-CC013-Human Papillomavirus (28 Types) Genotyping Detection Kit (Fluorescence PCR)
Epidemiology
Cervical cancer is one of the most common malignant tumors of female reproductive tract. Previous studies have shown that persistent infection and multiple infection of human papillomavirus is one of the important causes of cervical cancer. At present, there is still a lack of recognized effective treatment means for HPV, so early detection and early prevention of cervical HPV are the keys to block cancer. To establish a simple, specific and rapid etiological diagnosis method is of great significance in the clinical diagnosis of cervical cancer.
Channel
| Reaction buffer | FAM | VIC/HEX | ROX | CY5 |
| HPV Genotyping Reaction Buffer 1 | 16 | 18 | / | Internal control |
| HPV Genotyping Reaction Buffer 2 | 56 | / | 31 | Internal control |
| HPV Genotyping Reaction Buffer 3 | 58 | 33 | 66 | 35 |
| HPV Genotyping Reaction Buffer 4 | 53 | 51 | 52 | 45 |
| HPV Genotyping Reaction Buffer 5 | 73 | 59 | 39 | 68 |
| HPV Genotyping Reaction Buffer 6 | 6 | 11 | 83 | 54 |
| HPV Genotyping Reaction Buffer 7 | 26 | 44 | 61 | 81 |
| HPV Genotyping Reaction Buffer 8 | 40 | 43 | 42 | 82 |
Technical Parameters
| Storage | Liquid: ≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Cervical Swab、Vaginal Swab、Urine |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | 300Copies/mL |
| specificity | All results are negative when the kit is used to detect non-specific specimens that have cross reactions with it, including ureaplasma urealyticum, chlamydia trachomatis of reproductive tract, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella and other HPV types not covered by the kit. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
Recommended extraction reagents: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8)
Option 2.
Recommended extraction reagents: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)
