Human PML-RARA Fusion Gene Mutation
Product name
HWTS-TM017A Human PML-RARA Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Epidemiology
Acute promyelocytic leukemia (APL) is a special type of acute myeloid leukemia (AML). About 95% of APL patients are accompanied by a special cytogenetic change, namely t(15;17)(q22;q21), which makes the PML gene on chromosome 15 and the retinoic acid receptor α gene (RARA) on chromosome 17 fused to form the PML-RARA fusion gene. Due to different breakpoints of the PML gene, the PML-RARA fusion gene can be divided into long type (L type), short type (S type) and variant type (V type), accounting for approximately 55%, 40% and 5% respectively.
Channel
| FAM | PML-RARA fusion gene |
| ROX |
Internal Control |
Technical Parameters
| Storage |
≤-18℃ In dark |
| Shelf-life | 9 months |
| Specimen Type | bone marrow |
| CV | <5.0％ |
| LoD | 1000 Copies/mL. |
| Specificity | There is no cross-reactivity with other fusion genes BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, and TEL-AML1 fusion genes |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Recommended extraction reagent: RNAprep Pure Blood Total RNA Extraction Kit (DP433). The extraction should be conducted according to the IFU.
