Influenza A Virus/ Influenza B Virus
Product name
HWTS-RT174-Influenza A Virus/ Influenza B Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Based on the antigenic differences between NP gene and M gene, influenza viruses can be divided into four types: influenza A virus (IFV A), influenza B virus (IFV B), influenza C virus (IFV C) and influenza D virus (IFV D)[1]. Influenza A virus has many hosts and complex serotypes, and can acquire the ability to spread across hosts through genetic recombination and adaptive mutations. Humans lack lasting immunity to influenza A virus, so people of all ages are generally susceptible. Influenza A virus is the main pathogen causing influenza pandemics[2]. Influenza B virus is mostly prevalent in a small area and currently has no subtypes. The main ones that cause human infection are the B/Yamagata lineage or the B/Victoria lineage. Among the confirmed cases of influenza in 15 countries in the Asia-Pacific region each month, the confirmed rate of influenza B virus is 0-92%[3]. Unlike influenza A virus, specific groups such as children and the elderly are susceptible to influenza B virus and are prone to complications, which imposes a greater burden on society than influenza A virus[4].
Channel
| FAM | MP nucleic acid |
| ROX |
Internal Control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Oropharyngeal swab sample |
| Ct | Flu A, Flu B Ct≤35 |
| CV | <5.0% |
| LoD | Flu A and Flu B are all 200Copies/mL |
| Specificity |
Cross-reactivity: There is no cross reaction between the kit and Bocavirus, rhinovirus, cytomegalovirus, respiratory syncytial virus, parainfluenza virus, Epstein-Barr virus, herpes simplex virus, Varicella-zoster virus, mumps virus, enterovirus, measles virus, human metapneumovirus, adenovirus, human coronavirus, novel coronavirus, SARS coronavirus, MERS coronavirus, rotavirus, norovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis carinii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, Neisseria gonorrhoeae, Candida albicans, Candida glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium, and human genomic DNA. Interference test: Select mucin (60 mg/mL), human blood (50%), phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (20mg/mL) with 5% preservative, beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L) for interference tests, and the results show that the interfering substances at the above concentrations do not interfere with the detection of the kit. |
| Applicable Instruments | SLAN-96P Real-Time PCR Systems
Applied Biosystems 7500 Real-Time PCR System QuantStudio®5 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. are recommended for the sample extraction and the subsequent steps should be conducted in strict accordance with the IFU of the Kit.
