Influenza A Virus Universal/H1/H3
Product name
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit(Fluorescence PCR)
Epidemiology
Influenza virus is a representative species of Orthomyxoviridae. It is a pathogen that seriously threatens human health. It can infect the host extensively. The seasonal epidemic affects about 600 million people worldwide and causes 250,000 ~500,000 deaths, of which influenza A virus is the main cause of infection and death. Influenza A virus is a single-stranded negative-stranded RNA. According to its surface hemagglutinin (HA) and neuraminidase (NA), HA can be divided into 16 subtypes, NA Divided into 9 subtypes. Among influenza A viruses, the subtypes of influenza viruses that can directly infect humans are: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 and H10N8. Among them, H1 and H3 subtypes are highly pathogenic, and are particularly worthy of attention.
Channel
| FAM | influenza A universal type virus nucleic acid |
| VIC/HEX | influenza A H1 type virus nucleic acid |
| ROX | influenza A H3 type virus nucleic acid |
| CY5 | internal control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 9 months |
| Specimen Type | nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Copies/μL |
| Specificity |
No cross-reactivity with other respiratory samples such as Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. and human genomic DNA. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Nucleic Acid Extraction or Purification Reagent (YDP315-R) by Tiangen Biotech (Beijing) Co.,Ltd. The extraction should be carried out strictly according to the instructions for use. The extracted sample volume is 140μL, and the recommended elution volume is 60μL.
Option 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). The extraction should be carried out strictly according to the instructions for use. The extracted sample volume is 200μL, and the recommended elution volume is 80μL.
