Influenza A/B
Product name
HWTS-RT003A Influenza A/B Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Influenza A virus is an acute respiratory infectious disease, with multiple subtypes such as H1N1 and H3N2, which are prone to mutation and broke out worldwide. Antigenic shift refers to the mutation of the influenza A virus, resulting in the emergence of a new subtype. Influenza B viruses are divided into two major lineages, Yamagata and Victoria. Influenza B viruses only have antigenic drift, and they evade the surveillance and clearance of the human immune system through their mutations. However, the evolution rate of influenza B virus is slower than that of influenza A virus, and influenza B virus can also cause human respiratory tract infection and lead to epidemics.
Channel
| FAM | IFV A |
| ROX |
Internal Control |
| VIC/HEX | IFV B |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 9 months |
| Specimen Type | oropharyngeal swab |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | IFV A：500Copies/mL，IFV B：500Copies/mL |
| Specificity | 1. Cross-reactivity: there is no cross reactivity between this kit and adenovirus type 3, 7, human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, and HCoV-NL63, cytomegalovirus, enterovirus, parainfluenza virus, measles virus, human metapneumovirus, mumps virus, respiratory syncytial virus type B, rhinovirus, bordetella pertussis, chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus influenzae, jactobacillus, moraxella catarrhalis, avirulent mycobacterium tuberculosis, mycoplasma pneumoniae, neisseria meningitidis, neisseria gonorrhoeae, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, streptococcus salivarius and human genomic DNA.
2. Interference test: The interfering substances are selected as mucin (60mg/mL), human blood, oxymetazoline (2mg/mL), sulfur (10%), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), azithromycin (1mg/L), cephalosporin (40μg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir phosphate (60ng/mL), ribavirin (10mg/L), and the results show that interfering substances with the concentrations above have no interfering reaction to the detection of the kit. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System and BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
Recommended extraction reagents: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The extraction should be conducted according to the IFU. The extraction sample volume is 200μL. The recommended elution volume is 80μL.
Option 2.
Recommended extraction reagents: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). The extraction should be conducted according to the IFU.
Option 3.
Recommended extraction reagents: Nucleic Acid Extraction or Purification Kit (YDP315-R). The extraction should be conducted according to the IFU. The extraction sample volume is 140μL. The recommended elution volume is 60μL.
