Low MOQ for Mycobacterium Tuberculosis - Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR) – Macro & Micro-Test

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Low MOQ for Mycobacterium Tuberculosis - Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

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Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR)
Freeze-dried Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR)

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Epidemiology

Mycobacterium culosis is referred to as Tubercle bacillus(TB). Mycobacterium tuberculosis that is pathogenic to humans is now generally considered to be of human, bovine, and African types. Its pathogenicity may be related to the inflammation caused by the proliferation of bacteria in tissue cells, the toxicity of bacterial components and metabolites, and the immune damage to the bacterial components. Pathogenic substances are associated with capsules, lipids and proteins.

Mycobacterium tuberculosis can invade susceptible organisms through the respiratory tract, digestive tract or skin injury, causing tuberculosis of various tissues and organs, of which the most common is pulmonary tuberculosis through the respiratory tract. It usually occurs in children, and presents with symptoms such as low-grade fever, night sweats, and a small amount of hemoptysis. Secondary infection is mainly manifested as low-grade fever, night sweats, and hemoptysis. Mostly it is long-term chronic disease. In 2018, about 10 million people around the world were infected with Mycobacterium tuberculosis, of which about 1.6 million died.

Features

● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 10⁰ bacteria/mL.
● High specificity: No cross-reactivity with the human genome and other non-Mycobacterium tuberculosis and pneumonia pathogens.
● Convenient: One test for multiple results.

Channel

FAM: Target (IS6110 and 38KD) nucleic acid DNA
VIC (HEX): Internal control

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	2mins	No
2	1 cycle	95℃	10mins	No
3	40 cycles	95℃	15s	No
3	40 cycles	60℃	40s	Yes

Technical Parameters

Storage
Liquid	≤-18℃ In dark
Lyophilization	≤30℃
Shelf-life	12 months
Specimen Type	Sputum
Ct	≤39
CV	≤5.0％
LoD	10⁰ bacteria /mL

Specificity: No cross-reactivity with the human genome and other non-Mycobacterium tuberculosis and pneumonia pathogens.

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
ABI 7500 Fast Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.
BioRad CFX96 Real-Time PCR System, BioRad.
CFX Opus 96 Real-Time PCR System.

Main Components

Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR)

Component	Catalogue Number		Quantity	Component Description
	HWTS-RT001A	HWTS-RT001B
	Specification(50 tests/kit)	Specification(20 tests/kit)
TB Reaction Buffer	1 mL/vial	400μL/vial	1 vial	Mycobacterium tuberculosis IS6110 and 38KD, internal control specific primers, fluorescent probes, Taq enzyme, UDG enzyme, reaction buffer, etc.
TB Positive Control	1 mL/vial	400μL/vial	1 vial	IS6110, 38KD and internal control mixed plasmid diluent
TB Blank Control	1 mL/vial	400μL/vial	1 vial	DNase/RNase free H₂O

Freeze-dried Mycobacterium Tuberculosis DNA Detection Kit (Fluorescence PCR)

Component	Character	Catalogue Number		Component Description
		HWTS-RT105A	HWTS-RT105B
		Specification/Quantity(50 tests/kit)	Specification/Quantity(20 tests/kit)
TB Reaction Buffer	lyophilized	1 bottle	1 bottle	Contains Mycobacterium tuberculosis IS6110 and 38KD, internal control specific primers, fluorescent probes, Taq enzyme, UDG enzyme, reaction buffer, etc.
TB Positive Control	lyophilized	1 vial	1 vial	Contains IS6110, 38KD and internal control mixed plasmid diluent
TB Blank Control	Liquid	1 vial, 600μL/vial	1 vial, 600μL/vial	DNase/RNase free H₂O
Reconstituted Solution	Liquid	1 vial, 1.45mL/vial	1 vial, 1mL/vial	DNase/RNase free H₂O, etc.

Total PCR Solution

Option 1.

Mycobacterium Tuberculosis DNA Detection Kit7

Option 2.

Mycobacterium Tuberculosis DNA Detection Kit8

Reference

[1]. Programme W. Global tuberculosis control: WHO report [annual].[J]. Who Global Tuberculosis Programme, 2003.
[2]. Kocagoz T, Yilmaz E, Ozkara S, et al. Detection of Mycobacterium tuberculosis in sputum samples by polymerase chain reaction using a simplified procedure.[J]. Journal of clinical microbiology, 1993, 31(6):1435-1438.
[3]. Mikhailovich V, Lapa S, Gryadunov D, et al. Identification of Rifampin-Resistant Mycobacterium tuberculosis Strains by Hybridization, PCR, and Ligase Detection Reaction on Oligonucleotide Microchips[J]. Journal of Clinical Microbiology, 2001, 39(7):2531-2540.
[4]. Miller M B, Popowitch E B, Backlund M G, et al. Performance of Xpert MTB/RIF RUO Assay and IS6110 Real-Time PCR for Mycobacterium tuberculosis Detection in Clinical Samples[J]. Journal of Clinical Microbiology, 2011, 49(10):3458-62.

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Mycobacterium Bacteria