Middle East Respiratory Syndrome Coronavirus Nucleic Acid
Product name
HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)
Epidemiology
Middle East respiratory syndrome coronavirus (MERS-CoV), a β-coronavirus that causes respiratory disease in humans, was first identified in a deceased 60-year-old Saudi Arabian male patient on 24th July, 2012. The clinical presentation of MERS-CoV infection ranges from asymptomatic state or mild respiratory symptoms to severe acute respiratory disease even death.
Channel
| FAM | MERS virus RNA |
| VIC(HEX) |
Internal Control |
Technical Parameters
|
Storage |
≤-18℃ In dark |
|
Shelf-life |
9 months |
|
Specimen Type |
Freshly collected nasopharyngeal swabs |
|
CV |
≤5.0% |
|
Ct |
≤38 |
|
LoD |
1000 Copies/mL |
|
Specificity |
There is no cross-reactivity with human coronaviruses human coronavirus SARSr-CoV and other common pathogens. |
|
Applicable Instruments: |
Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
Recommended extraction reagent: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) by Tiangen Biotech (Beijing) Co., Ltd.
Option 2.
Recommended extraction reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
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