Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test

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Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Orthopox Virus Universal Type/Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

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Epidemiology

Monkeypox (MP) is an acute zoonotic infectious disease caused by Monkeypox Virus (MPV). The disease is mainly transmitted by animals, and humans could become infected by being bitten by infected animals or by direct contact with the blood, body fluids and rash of infected animals. The virus can also be transmitted between people, primarily through respiratory droplets during prolonged, direct face-to-face contact or through direct contact with a patient’s body fluids or contaminated objects^[1-2].

The clinical symptoms of monkeypox infection in humans are similar to those of smallpox, generally after a 12-day incubation period, appearing fever, headache, muscle and back pain, enlarged lymph nodes, fatigue and discomfort. The rash appears after 1-3 days of the fever, usually first on the face, but also in other parts. The disease course generally lasts 2-4 weeks, and the mortality rate is 1%-10%. Lymphadenopathy is one of the main differences between this disease and smallpox^[3].

Features

● Multiplex PCR Amplification Technology
● Accuracy: Internal control, fully monitor the experimental process to ensure the quality of Experiments.
● Rapid: Rapid PCR amplification shorts detection time, resulted in 40 minutes
● High sensitivity: 200 Copies/mL
● Triple PCR assay: Dual target gene(MPV-1 & MPV-2) of Monkeypox virus + human endogenous gene

Channel

Channel	Monkeypox	Monkeypox & Orthopox
FAM	Monkeypox virus MPV-1 gene	Orthopox virus universal type nucleic acid
VIC/HEX	Monkeypox virus MPV-2 gene	Monkeypox virus MPV-2 gene
ROX	/	Monkeypox virus MPV-1 gene
CY5	Internal Control	Internal control

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	2mins	No
2	1 cycle	95℃	1min	No
3	40 cycles	95℃	5s	No
3	40 cycles	55℃	15s	Yes

Technical Parameters

Storage	≤-18℃ in dark
Shelf-life	2 months
Specimen Type	Rash Fluid, Nasopharyngeal Swab, Throat Swab, Serum
Ct	≤38
CV	≤5.0％
LoD	200Copies/mL
Specificity
No cross-reactivity with Smallpox virus, Cowpox virus, Vaccinia virus, Herpes simplex virus, etc.
No cross-reactivity with other pathogens that cause rash disease.
No cross-reactivity with human genomic DNA.
Applicable Instruments	It can match the mainstream fluorescent PCR instruments on the market.
QuantStudio®5 Real-Time PCR Systems
SLAN-96P Real-Time PCR Systems
LightCycler®480 Real-Time PCR Systems
LineGene 9600 Plus Real-Time PCR Detection Systems
MA-6000 Real-Time Quantitative Thermal Cycler
BioRad CFX96 Real-Time PCR Systems
BioRad CFX Opus 96 Real-Time PCR Systems

Main Components

Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Catalogue Number	Component (50 tests/kit)	Specification	Quantity	Component Description
HWTS-OT071A	MPV Reaction Buffer	1.25mL /vial	1 vial	Amplification reaction reagents, monkeypox virus MPV-1 and MPV-2 primer probes and internal control primer probes, etc..
	MPV Positive Control	250μL/vial	1 vial	Monkeypox virus and internal control mixed plasmid
	MPV Blank Control	250μL/vial	1 vial	DNase/RNase free H₂O

Orthopox Virus Universal Type/Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Catalogue Number	Component (50 tests/kit)	Specification	Quantity	Component Description
HWTS-OT072A	OPV/MPV Reaction Buffer	1.25mL /vial	1 vial	Amplification reaction reagents, orthopox virus universal type and monkeypox virus primer probes, internal control primer probes, etc.
	OPV/MPV Positive Control	250μL/vial	1 vial	Orthopox virus universal type, monkeypox virus and internal control mixed plasmid
	OPV/MPV Blank Control	250μL/vial	1 vial	DNase/RNase free H₂O

Total PCR Solution

Option 1.

Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)8

Option 2.

Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)9

References

[1] Centers for Disease Control and Prevention. Human monkeypox-Kasai Oriental, Zaire, 1996-1997.MMWR. 1997, 46:304-307.
[2] Beer EM, Rao VB. A systematic review of the epidemiology of human monkeypox outbreaks and implications for outbreak strategy. PLoS Negl Trop Dis 2019 10;13(10).
[3] Jezek Z, Marennikova SS, Mutumbo M, et al. Human monkeypox: a study of 2,510 contacts of 214 patients. J Infect Dis 1986 Oct;154(4).

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Monkeypox Detection Kit