This kit is used for the in vitro qualitative detection of Mycobacterium tuberculosis DNA in human sputum, solid culture (L-J Medium) and liquid culture (MGIT Medium), bronchial lavage fluid, and the mutations in the 507-533 amino acid codon region (81bp, rifampicin resistance determining region) of the rpoB gene of Mycobacterium tuberculosis rifampicin resistance, as well as the mutations in the main mutation sites of Mycobacterium tuberculosis isoniazid resistance.It provides aid to the diagnosis of Mycobacterium tuberculosis infection, and it detects the main resistance genes of rifampicin and isoniazid, which helps to understand the drug resistance of Mycobacterium tuberculosis infected by the patient.