Mycobacterium Tuberculosis INH Mutation
Product name
HWTS-RT137 Mycobacterium Tuberculosis INH Mutation Detection Kit (Melting Curve)
Epidemiology
Mycobacterium tuberculosis, shortly as Tubercle bacillus (TB), is the pathogenic bacterium that causes tuberculosis. Currently, the commonly used first-line anti-tuberculosis drugs include INH, rifampicin and hexambutol, etc. The second-line anti-tuberculosis drugs include fluoroquinolones, amikacin and kanamycin, etc. The new developed drugs are linezolid, bedaquiline and delamani, etc. However, due to the incorrect use of anti-tuberculosis drugs and the characteristics of the cell wall structure of mycobacterium tuberculosis, mycobacterium tuberculosis develops drug resistance to anti-tuberculosis drugs, which brings serious challenges to the prevention and treatment of tuberculosis.
Channel
| FAM | MP nucleic acid |
| ROX |
Internal Control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | sputum |
| CV | ≤5% |
| LoD | The detection limit for wild-type INH bacteria is 2x103 bacteria/mL, and the detection limit for mutant bacteria is 2x103 bacteria/mL. |
| Specificity | a. There is no cross reaction among human genome, other nontuberculous mycobacteria and pneumonia pathogens detected by this kit.b. The mutation sites of other drug resistant genes in wild-type Mycobacterium tuberculosis, such as the resistance determining region of the rifampicin rpoB gene, were detected, and the test results showed no resistance to INH, indicating no cross reactivity. |
| Applicable Instruments | SLAN-96P Real-Time PCR SystemsBioRad CFX96 Real-Time PCR SystemsLightCycler480® Real-Time PCR System |
Work Flow
If use the Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. for extraction, add 200μL of the negative control and processed sputum sample to be tested in sequence, and add 10μL of the internal control separately into the negative control, processed sputum sample to be tested, and the subsequent steps should be strictly carried out according to the extraction instructions. The extracted sample volume is 200μL, and the recommended elution volume is 100μL.
