Mycoplasma Hominis Nucleic Acid

Short Description:

This kit is suitable for qualitative detection of Mycoplasma hominis (MH) in male urinary tract and female genital tract secretion samples.

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Product Detail

Product Tags

Product name

HWTS-UR004A-Mycoplasma Hominis Nucleic Acid Detection Kit (Fluorescence PCR)

Epidemiology

Sexually transmitted diseases (STDs) are still one of the important threats to global public health security, which can lead to infertility, premature fetal birth, tumorigenesis and various serious complications. Mycoplasma hominis exists in the genitourinary tract and can cause inflammatory responses in the genitourinary tract. The MH infection of the genitourinary tract can cause diseases like non-gonococcal urethritis, epididymitis, etc., and among females, which may cause inflammation of the reproductive system that spreads centered on the cervix. At the same time, the common complication of MH infection is salpingitis, and a small number of patients may have endometritis and pelvic inflammatory disease.

Channel

FAM	MH target
VIC(HEX)	Internal Control

Technical Parameters

Storage	Liquid: ≤-18℃ In dark
Shelf-life	12 months
Specimen Type	urethral secretions, cervical secretions
Ct	≤38
CV	＜5.0%
LoD	1000Copies/mL
Specificity	There is no cross-reactivity with other STD infection pathogens, which are outside the detection range, and there is no cross-reactivity with chlamydia trachomatis, ureaplasma urealyticum, neisseria gonorrhoeae, mycoplasma genitalium, herpes simplex virus type 1, herpes simplex virus type 2, etc.
Applicable Instruments	It can match the mainstream fluorescent PCR instruments on the market. Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). The extraction should be conducted in strict accordance with the instructions.

Option 2.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The extraction should be strictly conducted according to the instructions. The recommended elution volume should be 80 μL.

Option 3.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent (YDP302) by Tiangen Biotech (Beijing) Co., Ltd.. The extraction should be conducted in strict accordance with the instructions and the recommended elution volume is 80µL.

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