Mycoplasma Pneumoniae Nucleic Acid
Product name
HWTS-RT124A-Freeze-dried Mycoplasma Pneumoniae Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)
HWTS-RT129A-Mycoplasma Pneumoniae Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)
Certificate
CE
Epidemiology
Mycoplasma pneumoniae (MP) is the smallest prokaryotic microorganism with a cell structure and no cell wall between bacteria and viruses. MP mainly causes respiratory tract infections in humans, especially in children and young people. MP can cause Mycoplasma hominis pneumonia, respiratory tract infections in children and atypical pneumonia. The clinical symptoms are diverse, mostly severe cough, fever, chills, headache, sore throat, upper respiratory tract infection and bronchopneumonia are the most common. Some patients may develop severe pneumonia from upper respiratory tract infection, and severe respiratory distress or even death may occur. MP is one of the common and important pathogens in community-acquired pneumonia (CAP), accounting for 10%-30% of CAP, and the proportion can increase 3-5 times when MP is prevalent. In recent years, the proportion of MP in CAP pathogens has gradually increased. The incidence of Mycoplasma pneumoniae infection has increased, and because of its nonspecific clinical manifestations, it is easy to be confused with bacterial and viral colds. Therefore, early laboratory detection is of great significance for clinical diagnosis and treatment.
Channel
FAM | MP nucleic acid |
ROX |
Internal Control |
Technical Parameters
Storage |
Liquid: ≤-18℃ In dark, Lyophilized: ≤30℃ In dark |
Shelf-life | Liquid: 9 months, Lyophilized: 12 months |
Specimen Type | Throat swab |
Tt | ≤28 |
CV | ≤10.0% |
LoD | 2 Copies/μL |
Specificity |
No cross-reactivity with other respiratory samples such as Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. and human genomic DNA. |
Applicable Instruments |
Applied Biosystems 7500 Real-Time PCR Systems SLAN ®-96P Real-Time PCR Systems LightCycler® 480 Real-Time PCR system Easy Amp Real-time Fluorescence Isothermal Detection System(HWTS1600) |
Work Flow
Option 1.
Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Nucleic Acid Extractor(HWTS-3006).
Option 2.
Recommended extraction reagent: Nucleic Acid Extraction or Purification Kit(YD315-R) manufactured by Tiangen Biotech(Beijing) Co., Ltd.