EasyAmp by Macro & Micro Test—-A Portable Isothermal Fluorescence Amplification Instrument Compatible with LAMP/RPA/NASBA/HDA

Excellent Performance & Wide Application

Easy Amp, by the technology of isothermal nucleic acid amplification is featured with high sensitivity and short reaction period without requirements for temperature changing process. Therefore, it has emerged as the most favored technology for rapid nucleic acid testing. Without complicated thermal cycling systems, a simple and portable isothermal amplification device can satisfy the entire reaction process.

Macro & Micro-Test has independently developed and launched the EasyAmp, a portable fluorescent isothermal amplification instrument for various applications.

Currently, the Easy Amp has been validated to be compatible with several isothermal amplification techniques: Loop-Mediated Isothermal Amplification(LAMP), Recombinase Polymerase Amplification (RPA/RAA/MIRA), Nucleic Acid Sequence Based Amplification(NASBA), and Helicase Dependent Amplification(HDA). It shall be another cutting-edge instrument for rapid nucleic acid testing with much wider applications.

· Loop-Mediated Isothermal Amplification (LAMP): Amplification at 60-65°C with whole reaction process completed within 30 minutes. Seeing Figure 1 for its amplification principle:

 

Fig 1. Principle of LAMP  

· Recombinase Polymerase Amplification (RPA/RAA/MIRA): Amplification at 37°C-42°C with whole reaction process completed in 10-30 minutes. Seeing Figure 2 for its amplification principle:

Fig 2. Principle of Recombinase Polymerase Amplification 

· Nucleic Acid Sequence Based Amplification (NASBA): Amplification at 42°C with whole reaction process completed in 40-120 minutes. Seeing Figure 3 for its amplification principle:

Fig 3. Principle of Nucleic Acid Sequence Based Amplification 

· Helicase Dependent Amplification (HDA): Amplification at 65°C with whole reaction process completed in 70-90 minutes. Seeing Figure 4 for its amplification principle:

Fig4. Principle of Helicase Dependent Amplification 

· The EasyAmp enables efficient and stable amplification for DNA and RNA detection using the aforementioned four isothermal techniques. It achieves amplification sensitivity up to 200 copies/mL and ensures high specificity without cross-reactivity in target amplification.(Illustrated in Figures 5 to 7).

DNA Detection                                   RNA Detection

Fig5. RAA/MIRA Platform

DNA Detection                                   RNA Detection

Fig6. LAMP Platform

 

DNA Detection                                   RNA Detection

Fig7. NASBA Platform

Product Advantages:

Rapid

· EPIA technology realizes positive result detected in 5 min.

· Easy operation with one-step extraction for amplication on instrument.

Efficient

· 4 independent modules for on-demand, simultaneous testing of 4 different testing types.

· Each module capable of analyzing four samples concurrently.

· Dual/Quad fluorescence channels for extensive multi-target detection.

Compatibility

· Compatible with multiple isothermal platforms such as LAMP, RPA, NASBA, HDA.

· Designed for high-temperature amplification post low-temperature reverse transcription.

· Customizable to meet specific client needs.

User-Friendly

· Portable design (290×245×128mm, 2kg) for various application senarios.

· 7-inch color touchscreen for easy opeartion and vivid result illustration.

· Built-in thermal printer for immediate result printing.

· Integrated scanner for automatic detection item reading.