Nucleic Acid Detection Kit based on Enzymatic Probe Isothermal Amplification (EPIA) for Group B Streptococcus – Macro & Micro-Test

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Nucleic Acid Detection Kit based on Enzymatic Probe Isothermal Amplification (EPIA) for Group B Streptococcus – Macro & Micro-Test Detail:

Product Name

HWTS-UR019A-Freeze-dried Chlamydia Trachomatis, Ureaplasma Urealyticum and Neisseria Gonorrhoeae Nucleic Acid Detection Kit (Fluorescence PCR)

HWTS-UR019D-Chlamydia Trachomatis, Ureaplasma Urealyticum and Neisseria Gonorrhoeae Nucleic Acid Detection Kit (Fluorescence PCR)

Intended Use

This kit is suitable for the qualitative detection of common pathogens in urogenital infections in vitro, including Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU), and Neisseria gonorrhoeae (NG).

Epidemiology

Sexually transmitted diseases (STD) remain one of the major threats to global public health security, which can lead to infertility, premature fetal birth, tumorigenesis and various serious complications. There are many kinds of STD pathogens, including types like bacteria, viruses, chlamydia, mycoplasma and spirochetes, etc., and the common species are Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Herpes simplex virus type 1, herpes simplex virus type 2, Mycoplasma hominis, Mycoplasma genitalium, etc.

Channel

FAM: Chlamydia trachomatis (CT)

VIC(HEX): Ureaplasma urealyticum (UU)

ROX: Neisseria gonorrhoeae (NG)

CY5: Internal Control

PCR Amplification Conditions Setting

Liquid product:

Step

Cycles

Temperature

Time

Collect Fluorescent Signals or Not

1

1 cycle

50℃

5mins

No

2

1 cycle

95℃

10mins

No

3

40 cycles

95℃

15 secs

No

58℃

31 secs

Yes

Lyophilized product:

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 5mins No
2 1 cycle 95℃ 10mins No
3 40 cycles 95℃ 15 secs No
58℃ 30 secs Yes

Technical Parameters

Storage:

Liquid: ≤-18℃ In dark; Lyophilized: ≤30℃ In dark

Shelf-life: 12 months

Specimen Type: Urethral secretions, cervical secretions

Ct: ≤38

CV: ≤5.0%

LoD:

Liquid: 50 Copies/reaction; Lyophilized: 500Copies/mL

Specificity: There is no cross-reactivity for detecting other STD-infected pathogens, such as Treponema pallidum, etc.

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR System

QuantStudio® 5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd.


Product detail pictures:

Nucleic Acid Detection Kit based on Enzymatic Probe Isothermal Amplification (EPIA) for Group B Streptococcus – Macro & Micro-Test detail pictures


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Sticking to your belief of Creating solutions of high quality and generating buddies with people from all around the world, we always put the fascination of customers to start with for Nucleic Acid Detection Kit based on Enzymatic Probe Isothermal Amplification (EPIA) for Group B Streptococcus – Macro & Micro-Test , The product will supply to all over the world, such as: Romania, Thailand, Sri Lanka, With the support of our highly experienced professionals, we manufacture and supply best quality products. These are quality tested at various occasions to ensure only flawless range is delivered to customers, we also customize the array as per the need of customers to meet the requirement of customers.
  • We have been appreciated the Chinese manufacturing, this time also did not let us disappoint,good job!
    5 Stars By Helen from Sri Lanka - 2017.09.09 10:18
    The company can think what our think, the urgency of urgency to act in the interests of our position, can be said this is a responsible company, we had a happy cooperation!
    5 Stars By Ivy from Nigeria - 2017.12.02 14:11
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