Nucleic Acid Detection Kit based on Enzymatic Probe Isothermal Amplification (EPIA) for Group B Streptococcus – Macro & Micro-Test
Nucleic Acid Detection Kit based on Enzymatic Probe Isothermal Amplification (EPIA) for Group B Streptococcus – Macro & Micro-Test Detail:
Product Name
HWTS-UR019A-Freeze-dried Chlamydia Trachomatis, Ureaplasma Urealyticum and Neisseria Gonorrhoeae Nucleic Acid Detection Kit (Fluorescence PCR)
HWTS-UR019D-Chlamydia Trachomatis, Ureaplasma Urealyticum and Neisseria Gonorrhoeae Nucleic Acid Detection Kit (Fluorescence PCR)
Intended Use
This kit is suitable for the qualitative detection of common pathogens in urogenital infections in vitro, including Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU), and Neisseria gonorrhoeae (NG).
Epidemiology
Sexually transmitted diseases (STD) remain one of the major threats to global public health security, which can lead to infertility, premature fetal birth, tumorigenesis and various serious complications. There are many kinds of STD pathogens, including types like bacteria, viruses, chlamydia, mycoplasma and spirochetes, etc., and the common species are Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Herpes simplex virus type 1, herpes simplex virus type 2, Mycoplasma hominis, Mycoplasma genitalium, etc.
Channel
FAM: Chlamydia trachomatis (CT)
VIC(HEX): Ureaplasma urealyticum (UU)
ROX: Neisseria gonorrhoeae (NG)
CY5: Internal Control
PCR Amplification Conditions Setting
Liquid product:
Step |
Cycles |
Temperature |
Time |
Collect Fluorescent Signals or Not |
1 |
1 cycle |
50℃ |
5mins |
No |
2 |
1 cycle |
95℃ |
10mins |
No |
3 |
40 cycles |
95℃ |
15 secs |
No |
58℃ |
31 secs |
Yes |
Lyophilized product:
Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
1 | 1 cycle | 50℃ | 5mins | No |
2 | 1 cycle | 95℃ | 10mins | No |
3 | 40 cycles | 95℃ | 15 secs | No |
58℃ | 30 secs | Yes |
Technical Parameters
Storage:
Liquid: ≤-18℃ In dark; Lyophilized: ≤30℃ In dark
Shelf-life: 12 months
Specimen Type: Urethral secretions, cervical secretions
Ct: ≤38
CV: ≤5.0%
LoD:
Liquid: 50 Copies/reaction; Lyophilized: 500Copies/mL
Specificity: There is no cross-reactivity for detecting other STD-infected pathogens, such as Treponema pallidum, etc.
Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR System
QuantStudio® 5 Real-Time PCR Systems
SLAN-96P Real-Time PCR Systems
LightCycler®480 Real-Time PCR system
LineGene 9600 Plus Real-Time PCR Detection System
MA-6000 Real-Time Quantitative Thermal Cycler
BioRad CFX96 Real-Time PCR System
BioRad CFX Opus 96 Real-Time PCR System
Work Flow
Option 1.
Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).
Option 2.
Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd.
Product detail pictures:
Related Product Guide:
Our company puts emphasis on the management, the introduction of talented personnel, and the construction of staff building, trying hard to improve the quality and liability consciousness of staff members. Our company successfully attained IS9001 Certification and European CE Certification of Nucleic Acid Detection Kit based on Enzymatic Probe Isothermal Amplification (EPIA) for Group B Streptococcus – Macro & Micro-Test , The product will supply to all over the world, such as: belarus, Bangalore, Birmingham, Please feel cost-free to send us your specifications and we'll respond to you asap. We've got a professional engineering team to serve for the every single detailed needs. Free samples may be sent for you personally to know far more facts. So that you can meet your desires, please really feel cost-free to contact us. You could send us emails and call us straight. Additionally, we welcome visits to our factory from all over the world for much better recognizing of our corporation. nd merchandise. In our trade with merchants of several countries, we often adhere to the principle of equality and mutual advantage. It is our hope to market, by joint efforts, both trade and friendship to our mutual benefit. We look forward to getting your inquiries.
By Freda from Bulgaria - 2018.06.03 10:17
The goods we received and the sample sales staff display to us have the same quality, it is really a creditable manufacturer.
By Letitia from Palestine - 2017.11.29 11:09