Clostridium difficile toxin A/B gene（C.diff）
Product name
HWTS-OT031A Nucleic Acid Detection Kit for Clostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Certificate
CE
Epidemiology
Clostridium difficile (CD), a gram-positive anaerobic sporogenic Clostridium difficile, is one of the main pathogens causing nosocomial intestinal infections. Clinically, about 15%~25% of antimicrobial-related diarrhea, 50%~75% of antimicrobial-related colitis and 95%~100% of pseudomembranous enteritis are caused by Clostridium difficile infection (CDI). Clostridium difficile is a conditional pathogen, including toxigenic strains and non-toxigenic strains.
Channel
| FAM | tcdA gene |
| ROX | tcdB gene |
| VIC/HEX | Internal Control |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Specificity | use this kit to detect other intestinal pathogens such as Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus and human genomic DNA, the results are all negative. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System(FQD-96A，Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
Add 180μL of lysozyme buffer to the precipitate (dilute the lysozyme to 20mg/mL with lysozyme diluent), pipette to mix well, and process at 37°C for more than 30 minutes.Take 1.5mL of RNase/DNase-free centrifuge tube, and add 180μL of positive control and negative control in sequence. Add 10μL of internal control to the sample to be tested, positive control, and negative control in sequence, and use the Nucleic Acid Extraction or Purification Reagent (YDP302) by Tiangen Biotech (Beijing) Co., Ltd. for subsequent sample DNA extraction, and please strictly follow the instructions for use for specific steps. Use DNase/RNase free H2O for elution, and the recommended elution volume is 100μL.
Option 2.
Take 1.5mL of RNase/DNase-free centrifuge tube, and add 200μL of positive control and negative control in sequence. Add 10μL of internal control to the sample to be tested, positive control, and negative control in sequence, and use the Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). The extraction should be carried out in strict accordance with the instruction for use, and the recommended elution volume is 80μL.
