OEM China Hpv Cervix - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test
OEM China Hpv Cervix - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test Detail:
Product name
28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR )
Certificate
CE
Epidemiology
Cervical cancer is one of the most common malignant tumors in the female reproductive tract. The studies have shown that persistent infection and multiple infections of human papillomavirus is one of the important cause of cervical cancer. At present, there is still a lack of recognized effective treatment methods for HPV. Therefore, early detection and early prevention of cervical HPV is the key to blocking canceration. The establishment of a simple, specific and rapid pathogenic diagnostic method is of great significance in the clinical diagnosis of cervical cancer.
Features
● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 25 Copies/reaction
● High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.
● Convenient: One test for multiple results.
Channel
S/N | Channel | Type |
PCR-Mix1 | FAM | 16, 18, 31, 56 |
VIC(HEX) | Internal Control | |
CY5 | 45, 51, 52, 53 | |
ROX | 33, 35, 58, 66 | |
PCR-Mix2 | FAM | 6, 11, 54, 83 |
VIC(HEX) | 26, 44, 61, 81 | |
CY5 | 40, 42, 43, 82 | |
ROX | 39, 59, 68, 73 |
PCR Amplification Conditions Setting
Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
1 | 1 cycle | 50℃ | 2 mins | No |
2 | 1 cycle | 95℃ | 10 mins | No |
3 | 10 cycle | 95℃ | 15 s | No |
62℃ | 20 s | No | ||
4 | 30 cycle | 95℃ | 15 s | No |
58℃ | 30 s | Yes |
Technical Parameters
Storage | ≤-18℃ in dark |
Shelf-life | 12 months |
Specimen Type | Cervical exfoliated cells |
Ct | ≤28 |
CV | ≤5.0% |
LoD | 25 Copies/reaction |
Specificity | No cross-reactivity with common reproductive tract pathogens (such as ureaplasma urealyticum, genital tract chlamydia trachomatis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella and other HPV types not covered in the kit, etc). |
Applicable Instruments | It can match the mainstream fluorescent PCR instruments on the market. |
SLAN-96P Real-Time PCR Systems | |
ABI 7500 Real-Time PCR Systems | |
QuantStudio®5 Real-Time PCR Systems | |
LightCycler®480 Real-Time PCR Systems | |
LineGene 9600 Plus Real-Time PCR Detection Systems | |
MA-6000 Real-Time Quantitative Thermal Cycler |
Main Components
Catalogue Number | Component (50 tests/kit) | Specification | Quantity | Component Description |
HWTS-CC003A | HPV WELL1 | 1.3mL/vial | 1 vial | Mixture of specific primers and probes of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66 |
HPV WELL2 | 1.3mL/vial | 1 vial | Mixture of specific primers and probes of HPV6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83 | |
HPV Enzyme Mix | 55uL/vial | 1 vial | Mixture of Taq DNA polymerase, Uracil-DNA Glycosylase and glycerin | |
HPV Positive Control WELL1 | 150μL/vial | 1 vial | Mixture of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66 templates and Internal Control template | |
HPV Positive Control WELL2 | 150μL/vial | 1 vial | Mixture of 6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83 templates | |
HPV Blank Control | 300μL/vial | 1 vial | RNase/DNase free H2O |
Total PCR Solution
Option 1.
Option 2.
Product detail pictures:
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We not only will try our greatest to present fantastic expert services to each purchaser, but also are ready to receive any suggestion offered by our prospects for OEM China Hpv Cervix - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test , The product will supply to all over the world, such as: Leicester, Southampton, United States, Our company has always insisted on the business principle of Quality, Honest, and Customer First by which we have won the trust of clients both from at home and abroad. If you are interested in our products, please do not hesitate to contact us for further information.
By Gemma from Cape Town - 2017.04.18 16:45
This is a reputable company, they have a high level of business management, good quality product and service, every cooperation is assured and delighted!
By John biddlestone from Indonesia - 2018.02.04 14:13